000			03833nam a22003735i 4500
001			235227
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007			cr nn 008mamaa
008			160811s2016 xxu| s |||| 0|eng d
020			_a9781493937899
024	7		_a10.1007/978-1-4939-3789-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCilia _bMethods and Protocols _cedited by Peter Satir, Søren Tvorup Christensen.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XIV, 259 páginas) _b54 ilustraciones, 39 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1454
505	0		_aMethods for Studying Ciliary Import Mechanisms -- Targeting of ASH Domain-Containing Proteins to the Centrosome -- Morphological and Functional Characterization of the Ciliary Pocket by Electron and Fluorescence Microscopy -- Methods to Study Interactions between Ciliogenesis and Autophagy -- Recombinant Reconstitution and Purification of the IFT B Core Complex from Chlamydomonas reinhardtii -- Methods for Studying Movement of Molecules within Cilia -- A FRAP-Based Method for Monitoring Molecular Transport in Ciliary Photoreceptor Cells In Vivo -- Kymographic Analysis of Transport in an Individual Neuronal Sensory Cilium in Caenorhabditis elegans -- Visualization and Manipulation of Cilia and Intraciliary Calcium in the Zebrafish Left-Right Organizer -- Methods for Studying Ciliary-Mediated Chemoresponse in Paramecium -- STED and STORM Superresolution Imaging of Primary Cilia -- CLEM Methods for Studying Primary Cilia -- Methods for Visualization of Neuronal Cilia -- Methods to Study Centrosomes and Cilia in Drosophila -- Analysis of Axonemal Assembly during Ciliary Regeneration in Chlamydomonas -- Planaria as a Model System for the Analysis of Ciliary Assembly and Motility.
520			_aThis volume is the result of an explosion of molecular-based research on Cilia, which began with the discovery of the universality of intraflagellar transport (IFT) and ciliary genomics/proteomics. The chapters in this book cover topics such as: high resolution imaging and functional characterization of sensory and primary cilia in mammalian cells and zebrafish, methods to study ciliary-mediated chemoresponse in Paramecium, and methods to study centrosomes and cilia in C. elegans and Drosophila. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Cilia: Methods and Protocols is broad and covers motile, sensory, and primary cells. It is a valuable resource to anyone interested in entering the field of ciliary biology using model organisms, including flagellate algae, ciliates, planaria, nematodes, insects, zebrafish, and mammalian cells. .
700	1		_aSatir, Peter _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aChristensen, Søren Tvorup _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493937875
776	0	8	_iPrinted edition: _z9781493937882
776	0	8	_iPrinted edition: _z9781493981397
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3789-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c235227 _d235227