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001			235233
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008			170628s2017 xxu| s |||| 0|eng d
020			_a9781493969968
024	7		_a10.1007/978-1-4939-6996-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aLipidomics _bMethods and Protocols _cedited by Sanjoy K. Bhattacharya.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XII, 281 páginas) _b74 ilustraciones, 41 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1609
505	0		_aLipid Sample Preparation for Biomedical Research -- Lipid Extraction Techniques for Stable Isotope Analysis and Ecological Assays -- Isolation of Lipid Raft Proteins from CD133+ Cancer Stem Cells -- Isolation of Neuronal Synaptic Membranes by Sucrose Gradient Centrifugation -- Sample Preparation and Analysis for Imaging Mass Spectrometry -- Direct Measurement of Free and Esterified Cholesterol Mass in Differentiated Human Podocytes: A TLC and Enzymatic Assay Based Method -- High-Performance Chromatographic Separation of Cerebrosides -- Lipid Identification by Untargeted Tandem Mass Spectrometry Coupled with Ultra-High-Pressure Liquid Chromatography -- Utility of Moderate and High Resolution Mass Spectrometry for Class-Specific Lipid Identification and Quantification -- A Robust Lipidomics Workflow for Mammalian Cells, Plasma, and Tissue using Liquid-Chromatography High-Resolution Tandem Mass Spectrometry -- Combined Use of MALDI-TOF Mass Spectrometry and 31PNMR Spectrometry for Analysis of Phospholipids -- Global Monitoring of Mammalian Lipidome by Quantitative Shotgun Lipodomics -- Bioinformatics Pertinent to Lipid Analysis in Biological Samples -- LC-MS-Based Lipidomics and Automated Identification of Lipids using the LipidBlast In-Silico MS/MS Library -- Single-Step Capture and Targeted Metabolomics of Alkyl-Quinolones in Outer Membrane Vesicles of Pseudomonas aeruginosa -- Analysis of Fatty Acid and Cholesterol Content from Detergent-Resistant and Detergent-Free Membrane Microdomains -- Computational Functional Analysis of Lipid Metabolic Enzymes -- Isoprenylation of Monomeric GTPases in Human Trabecular Meshwork Cells -- Purification and Validation of Lipid Transfer Proteins -- Incorporation of Artificial Lipid-Anchored Proteins into Cultured Mammalian Cells -- Sonication-Based Basic Protocol for Liposome Synthesis -- On Electrochemical Methods for Determination of Protein-Lipid Interaction -- Angiogenesis Model of Cornea to Understand the Role of Sphingosine 1-Phosphate. .
520			_aThis volume explores lipidomics through protocols that focus on areas of utility, techniques, and bioinformatics advancements. The protocols in this book cover topics such as isolation of specific membranes and specialized fractionation of subcellular compartments, and computational and functional analysis of lipid metabolizing enzymes. Each protocol also discusses methods of dealing with fractionated organelles-cells to whole organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Lipidomics: Methods and Protocols is a useful and valuable resource for biochemists, molecular biologists, and neuroscientists interested in neurology, ophthalmology and vision sciences, as well as mass spectrometrists with interest in disease discovery. .
700	1		_aBhattacharya, Sanjoy K _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493969951
776	0	8	_iPrinted edition: _z9781493969975
776	0	8	_iPrinted edition: _z9781493983629
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6996-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c235233 _d235233