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001			235235
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007			cr nn 008mamaa
008			160203s2016 xxu| s |||| 0|eng d
020			_a9781493933600
024	7		_a10.1007/978-1-4939-3360-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aClinical Applications of PCR _cedited by Rajyalakshmi Luthra, Rajesh Singh, Keyur Pravinchandra Patel.
250			_a3rd edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (X, 191 páginas) _b25 ilustraciones, 16 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1392
505	0		_aA Targeted Q-PCR-Based Method for Point Mutation Testing by Analyzing Circulating DNA for Cancer Management Care -- COLD-PCR: Applications and Advantages -- PCR-Based Detection of DNA Copy Number Variation -- Emulsion PCR: Techniques and Applications -- Digital PCR: Principles and Applications -- Quantitative PCR for Plasma Epstein-Barr Virus Loads in Cancer Diagnostics -- High Resolution Melt Curve Analysis in Cancer Mutation Screen -- Locked Nucleic Acid Probes (LNA) for Enhanced Detection of Low-Level, Clinically Significant Mutations -- Genotyping of Frequent Mutations in Solid Tumors by PCR-Based Single-Base Extension and MassARRAY Analysis -- Microfluidics-Based PCR for Fusion Transcript Detection -- Polymerase Chain Reaction Diagnosis of Leishmaniasis: A Species-Specific Approach -- Detection of Trypanosoma cruzi by Polymerase Chain Reaction -- PCR Techniques in Next-Generation Sequencing -- Single Cell Quantitative PCR: Advances and Potential in Cancer Diagnostics -- Quantitative Real Time PCR: Recent Advances -- PCR Techniques in Characterizing DNA Methylation.
520			_aThis updated volume explores a wide variety of clinical applications of PCR such as detecting DNA methylation, detection of viruses and protozoa in infectious diseases, estimation of gene copy number aberrations, primer extension coupled with mass spectroscopy, and high throughput NGS techniques. The application of PCR has shown incredible value in the study of genomics and transcriptomics, not only for discovery but also for routine clinical applications, and it forms the cornerstone of personalized medicine. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and authoritative, Clinical Applications of PCR, Third Edition serves as an ideal guide for researchers aiming to understand the principles behind each application and for their implementation in the laboratory.
700	1		_aLuthra, Rajyalakshmi _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aSingh, Rajesh _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aPatel, Keyur Pravinchandra _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493933587
776	0	8	_iPrinted edition: _z9781493933594
776	0	8	_iPrinted edition: _z9781493980307
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3360-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c235235 _d235235