000			04237nam a22003615i 4500
001			235236
003			ES-VaUE
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007			cr nn 008mamaa
008			120117s2012 xxu| s |||| 0|eng d
020			_a9781617794988
024	7		_a10.1007/978-1-61779-498-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aProteoglycans _bMethods and Protocols _cedited by Françoise Rédini.
250			_a1st edition 2012
264		1	_aTotowa, NJ _bHumana Press _c2012
300			_a1 recurso en línea (XIV, 359 páginas) _b60 ilustraciones, 16 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v836
505	0		_aProteoglycans: Gene Cloning -- Proteoglycan: Site Mapping and Site-Directed Mutagenesis -- Mapping of the Wnt/β-Catenin/TCF Response Elements in the Human Versican Promoter -- Gene Silencing in Mouse Embryonic Stem Cells -- A Novel Strategy for a Splice-Variant Selective Gene Ablation: The Example of the Versican V0/V2 Knockout -- Detection of Neurocan in Cerebrospinal Fluid -- Glycosaminoglycan Chain Analysis and Characterization (Glycosylation/Epimerization) -- Characterization of Glycosaminoglycans by Tandem Vibrational Microspectroscopy and Multivariate Data Analysis -- Glycosaminoglycans: Oligosaccharide Analysis by Liquid Chromatography/Specific Labeling -- Brain Chondroitin/Dermatan Sulfate, from Cerebral Tissue to Fine Structure: Extraction, Preparation, and Fully Automated Chip-Electrospray Mass Spectrometric Analysis -- Use of Neutrons Reveal the Dynamics of Cell Surface Glycosaminoglycans -- Following Protein-Glycosaminoglycan (GAG) Polysaccharide Interactions with Differential Scanning Fluorimetry (DSF) -- In Vivo Scintigraphic Imaging of Proteoglycans -- Serglycin: The Master of the Mast Cell -- Analysis of Aggrecan Catabolism by Immunoblotting and Immunohistochemistry -- Heparan Sulfate Proteoglycans as Multifunctional Cell Regulators: Cell Surface Receptors -- Models for Studies of Proteoglycans in Kidney Pathophysiology -- Lumican Promotes Corneal Epithelial Wound Healing -- Shedding of Cell Membrane-Bound Proteoglycans -- Modulatory Effects of Proteoglycans on Proteinase Activities -- Proteoglycans and Osteolysis -- Proteoglycans and Cartilage Repair.
520			_aNow recognized as a reservoir for growth factors and cytokines modulating cell activation status and turnover, proteoglycans have stimulated great amount of interest and research.  In Proteoglycans: Methods and Protocols, experts in the field examine issues of basic concepts and up-to-date analysis methods for proteoglycan and glycosaminoglycan (GAG) at the protein and saccharide levels.  The volume continues with the multifunctional aspect of proteoglycans, highlighted through three relevant examples of proteoglycans with highly different strucutures: serglycin, aggrecan, and heparin sulphate proteoglycans.  The final chapter describes proteoglycan involvement in the pathogenesis of various disorders and their potential therapeutic value in osteo-articular diseases. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and accessible, Proteoglycans: Methods and Protocols is an ideal guide for scientists attempting to pursue further research in this vital and complex area of study.
700	1		_aRédini, Françoise _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617794971
776	0	8	_iPrinted edition: _z9781617794995
776	0	8	_iPrinted edition: _z9781493958795
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-498-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2012
999			_c235236 _d235236