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008			100301s2009 xxu| s |||| 0|eng d
020			_a9781597451963
024	7		_a10.1007/978-1-59745-196-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHigh Throughput Protein Expression and Purification _bMethods and Protocols _cedited by Sharon A. Doyle.
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XII, 322 páginas) _b57 ilustraciones, 6 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v498
505	0		_aHigh-Throughput Protein Production (HTPP): A Review of Enabling Technologies to Expedite Protein Production -- Designing Experiments for High-Throughput Protein Expression -- Gateway Cloning for Protein Expression -- Flexi Vector Cloning -- The Precise Engineering of Expression Vectors Using High-Throughput In-Fusion™ PCR Cloning -- The Polymerase Incomplete Primer Extension (PIPE) Method Applied to High-Throughput Cloning and Site-Directed Mutagenesis -- A Family of LIC Vectors for High-Throughput Cloning and Purification of Proteins -- "System 48" High-Throughput Cloning and Protein Expression Analysis -- Automated 96-Well Purification of Hexahistidine-Tagged Recombinant Proteins on MagneHis Ni2+-Particles -- E. coli and Insect Cell Expression, Automated Purification and Quantitative Analysis -- Hexahistidine-Tagged Maltose-Binding Protein as a Fusion Partner for the Production of Soluble Recombinant Proteins in Escherichia coli -- PHB-Intein-Mediated Protein Purification Strategy -- High-Throughput Biotinylation of Proteins -- High-Throughput Insect Cell Protein Expression Applications -- High-Throughput Protein Expression Using Cell-Free System -- The Production of Glycoproteins by Transient Expression in Mammalian Cells -- High-Throughput Expression and Detergent Screening of Integral Membrane Proteins -- Cell-Free Expression for Nanolipoprotein Particles: Building a High-Throughput Membrane Protein Solubility Platform -- Expression and Purification of Soluble His6-Tagged TEV Protease -- High-Throughput Protein Concentration and Buffer Exchange: Comparison of Ultrafiltration and Ammonium Sulfate Precipitation.
520			_aDespite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In High Throughput Protein Expression and Purification: Methods and Protocols, leading scientists detail the most successful protocols currently in use, including various high throughput cloning schemes, protein expression analysis, and production protocols. This volume describes the use of E. coli, insect, and mammalian cells, as well as cell-free systems for the production of a wide variety of proteins, including glycoproteins and membrane proteins, in order to best represent strategies that create and exploit common features to enable simplified cloning, stable expression, and purification of proteins. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section for tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, High Throughput Protein Expression and Purification: Methods and Protocols is an ideal reference for protein biochemists and all those who wish to apply these easy-to-use protocols to the many applicable fields.
700	1		_aDoyle, Sharon A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781603279758
776	0	8	_iPrinted edition: _z9781617378218
776	0	8	_iPrinted edition: _z9781588298799
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-196-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2009
999			_c235240 _d235240