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001			235243
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007			cr nn 008mamaa
008			140207s2014 xxu| s |||| 0|eng d
020			_a9781461496328
024	7		_a10.1007/978-1-4614-9632-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aElectroporation Protocols _bPreclinical and Clinical Gene Medicine _cedited by Shulin Li, Jeffry Cutrera, Richard Heller, Justin Teissie.
250			_a2nd edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XIV, 349 páginas) _b69 ilustraciones, 49 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1121
505	0		_aElectroporation-Based Gene Therapy: Recent Evolution in the Mechanism Description and Technology Developments -- Electropermeabilization of the Cell Membrane -- The Impact of Non-Electrical Factors on Electrical Gene Transfer -- Electroporation Formulation for Cell Therapy -- Short-Fragment DNA-Mediated in Vivo DNA Electroporation Delivery -- Gold Nanoparticle-Enhanced Electroporation for Leukemia Cell Transfection -- SiRNA Delivery via Electropulsation: A Review of the Basic Processes -- Continuous Cell Electroporation for Efficient DNA and siRNA Delivery Based on Laminar Microfluidic Chips -- Electroporation of siRNA into Mouse Bone Marrow-Derived Macrophages and Dendritic Cells -- Direct Imaging of siRNA Electrotransfer at the Single Cell Level -- Electroporation-Mediated siRNA Delivery into Tumors -- Substrate-Mediated, High Efficiency siRNA Electroporation -- Intradermal Electroporation of RNA -- Delivery of DNA into the Central Nervous System via Electroporation -- DNA Delivery in Adult Mouse Eyes: An Update with Corneal Outcomes -- Delivery of Plasmid DNA into Developing Rat Teeth by Electroporation -- Electroporation-Mediated Gene Delivery to the Lungs -- Electroporation-Mediated Delivery of Genes in Rodent Models of Lung Contusion -- In Vivo Electroporation-Mediated Gene Delivery to the Beating Heart -- Managing Local Swelling Following Intratumoral Electro-Chemo-Gene Therapy -- Gene Electrotransfer in Clinical Trials -- Electrochemotherapy in Veterinary Oncology: From Rescue to First-line Therapy -- Low Energy DC Current Ablation in a Mouse Tumor Model -- Evolution of Electroporated DNA Vaccines -- Clinical Development of Intramuscular Electroporation: Providing a "Boost" for DNA Vaccines -- Electroporation-Mediated Administration of Candidate DNA Vaccines against HIV-1 -- Electroporation-Mediated Intradermal Delivery of DNA Vaccines in Nonhuman Primates -- Intramuscular DNA Vaccination Protocols Mediated by Electric Fields Pieranna -- DNA Electroporation of Multi-Agent Vaccines Conferring Protection Against Select Agent Challenge-TriGrid Delivery System -- Gene-Based Vaccination and Screening Methods to Develop Monoclonal Antibodies.
520			_aElectroporation gene therapy, or gene electrotransfer, has evolved greatly over the last few decades as a result of the remarkable progress in genetic sequencing, gene array analysis, gene cloning, gene expression detection, DNA manufacture, and discovery and synthesis of siRNA. Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues, and for internal organs. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition aims to provide not only comprehensive coverage of the basic theory and practical application of electroporation siRNA therapy, gene therapy, and vaccine, but also elaborates on the most current views from the experts in this field, serving as an invaluable resource for investigators both in and outside of this field.
700	1		_aLi, Shulin _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aCutrera, Jeffry _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aHeller, Richard _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aTeissie, Justin _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781461496335
776	0	8	_iPrinted edition: _z9781461496311
776	0	8	_iPrinted edition: _z9781493954926
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4614-9632-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c235243 _d235243