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020 _a9781597452458
024 7 _a10.1007/978-1-59745-245-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aAdipose Tissue Protocols
_cedited by Kaiping Yang.
250 _a2nd edition 2008
264 1 _aTotowa, NJ
_bHumana Press
_c2008
300 _a1 recurso en línea (XVI, 336 páginas)
_b61 ilustraciones, 9 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v456
505 0 _aOverview of Adipose Tissue and Its Role in Obesity and Metabolic Disorders -- Choosing an Adipose Tissue Depot for Sampling -- Application of Imaging and Other Noninvasive Techniques in Determining Adipose Tissue Mass -- Generation of Adipose Tissue-Specific Transgenic Mouse Models -- Angiogenesis in Adipose Tissue -- Adipose Organ Nerves Revealed by Immunohistochemistry# -- Human Adipose Tissue Blood Flow and Micromanipulation of Human Subcutaneous Blood Flow -- Studies of Thermogenesis and Mitochondrial Function in Adipose Tissues -- Application of Lipidomics and Metabolomics to the Study of Adipose Tissue -- Applications of Proteomics to the Study of Adipose Tissue -- Application of DNA Microarray to the Study of Human Adipose Tissue/Cells -- Differentiation of Adipose Stem Cells -- Methods That Resolve Different Contributions of Clonal Expansion to Adipogenesis in 3T3-L1 and C3H10T1/2 Cells -- Explant Cultures of White Adipose Tissue -- Isolation and Culture of Preadipocytes from Rodent White Adipose Tissue -- Flow Cytometry on the Stromal-Vascular Fraction of White Adipose Tissue -- Application of Electrophoretic Mobility Shift Assay and Chromatin Immunoprecipitation in the Study of Transcription in Adipose Cells -- Application of RNA Interference Techniques to Adipose Cell Cultures -- RNA Isolation and Real-Time Quantitative RT-PCR -- Study of Adipose Tissue Gene Expression by In Situ Hybridization -- Application of Immunocytochemistry and Immunofluorescence Techniques to Adipose Tissue and Cell Cultures -- Determination of Lipolysis in Isolated Primary Adipocytes -- Study of Glucose Uptake in Adipose Cells -- Measurement of Phosphoinositide 3-Kinase and Its Products to Study Adipogenic Signal Transduction.
520 _aSince the publication of the first edition, the techniques and experimental approaches employed in the study of adipose tissue have undergone radical expansion and revision. In Adipose Tissue Protocols: Second Edition, several updated chapters are combined with many new contributions from internationally recognized experts covering both conventional and state-of-the-art methodologies as well as whole animal, cellular and molecular approaches which have emerged in or been extended to adipose tissue research over the past few years. As a volume in the highly sucessful Methods in Molecular Biology™ series, each chapter includes an introduction to its subject matter, a list of necessary materials, step-by-step, readily reproducible laboratory protocols, and a Notes section, which provides invaluable tips and tricks to safeguard against potential mishaps and pitfalls. Cutting-edge and comprehensive, Adipose Tissue Protocols: Second Edition is an essential manual for newcomers to this burgeoning area as well as a valuable addition to the laboratories and offices of more experienced researchers.
700 1 _aYang, Kaiping
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617378447
776 0 8 _iPrinted edition:
_z9781588299161
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-245-8
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2008
999 _c235247
_d235247