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008			140317s2014 xxu| s |||| 0|eng d
020			_a9781627037945
024	7		_a10.1007/978-1-62703-794-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMetalloproteins _bMethods and Protocols _cedited by Juan C. Fontecilla-Camps, Yvain Nicolet.
250			_a1st edition 2014
264		1	_aTotowa, NJ _bHumana Press _c2014
300			_a1 recurso en línea (X, 299 páginas) _b89 ilustraciones, 57 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1122
505	0		_aAdvances in Research on Metalloproteins -- Purification of O2-Sensitive Metalloproteins -- Expression and Purification of NifB Protein from Aerobic and Anaerobic Sources -- Techniques for the Production, Isolation and Analysis of Iron-Sulfur Proteins -- Cell-Free Synthesis of the H-cluster: a Model for the In Vitro Assembly of Metalloprotein Metal Centers -- Electrochemistry of Metalloproteins - Protein Film Electrochemistry for the Study of E. coli [NiFe]-Hydrogenase-1 -- FTIR Spectroscopy of Metalloproteins -- Characterizing Millisecond Intermediates in Hemoproteins Using Rapid-Freeze-Quench Resonance Raman Spectroscopy -- A Practical Guide for Nuclear Resonance Vibrational Spectroscopy (NRVS) of Biochemical Samples and Model Compounds -- Study of Metalloproteins Using Continuous Wave Electron Paramagnetic Resonance (EPR) -- Mössbauer Spectroscopy -- X-Ray Absorption Spectroscopy of Metalloproteins -- X-Ray Crystallographic Studies of Metalloproteins -- Quantum Mechanical Methods for the Investigation of Metalloproteins and Related Bioinorganic Compounds -- Density Functional Theory - Broken Symmetry (DFT-BS) Methodology Applied to Electronic and Magnetic Properties of Bioinorganic Prosthetic Groups.
520			_aMetalloproteins are involved in many key biological processes such as gas transport and metabolism, photosynthesis, cell respiration, the Krebs cycle and many other vital redox reactions. Metalloproteins: Methods and Protocols addresses multiple aspects of metalloenzyme research from the production and purification of metalloproteins using both standard recombinant techniques and a cell-free system, to the electrochemical analysis of metalloproteins, as well as IR techniques, Mössbauer spectroscopy and the theoretical interpretation of metalloenzyme catalytic and redox processes, to name a few topics. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Metalloproteins: Methods and Protocols seeks to serve both professionals and novices interested in the metalloprotein field.
700	1		_aFontecilla-Camps, Juan C _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aNicolet, Yvain _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627037952
776	0	8	_iPrinted edition: _z9781627037938
776	0	8	_iPrinted edition: _z9781493963287
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-794-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c235250 _d235250