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008			121026s2013 xxu| s |||| 0|eng d
020			_a9781627031370
024	7		_a10.1007/978-1-62703-137-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aNanoimaging _bMethods and Protocols _cedited by Alioscka A. Sousa, Michael J. Kruhlak.
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XIV, 510 páginas) _b132 ilustraciones, 76 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v950
505	0		_aIntroduction: Nanoimaging Techniques in Biology -- Live-Cell Imaging of Vesicle Trafficking and Divalent Metal Ions by Total Internal Reflection Fluorescence (TIRF) Microscopy -- 4Pi Microscopy -- Fluorescence in situ Hybridization Applications for Super-Resolution 3D Structured Illumination Microscopy -- Two-Color STED Imaging of Synapses in Living Brain Slices -- Super-Resolution Imaging by Localization Microscopy -- High-Content Super-Resolution Imaging of Live Cell by uPAINT -- Super-Resolution Fluorescence Imaging with Blink Microscopy -- Photoswitchable Fluorophores for Single-Molecule Localization Microscopy -- Single-Molecule Tracking of mRNA in Living Cells -- Semiautomatic, High-Throughput, High-Resolution Protocol for Three-Dimensional Reconstruction of Single Particles in Electron Microscopy -- Mass Mapping of Amyloid Fibrils in the Electron Microscope using STEM Imaging -- Elemental Mapping by Electron Energy Loss Spectroscopy in Biology -- Cellular Nanoimaging by Cryo Electron Tomography -- Large-Volume Reconstruction of Brain Tissue from High-Resolution Serial Section Images Acquired by SEM-Based Scanning Transmission Electron Microscopy -- 3D Imaging of Cells and Tissues by Focused Ion Beam/Scanning Electron Microscopy (FIB/SEM) -- Preparation of Gold Nanocluster Bioconjugates for Electron Microscopy -- Atomic Force Microscopy Imaging of Macromolecular Complexes -- Imaging of Transmembrane Proteins Directly Incorporated Within Supported Lipid Bilayers Using Atomic Force Microscopy -- Functional AFM Imaging of Cellular Membranes Using Functionalized Tips -- Near-Field Scanning Optical Microscopy for High Resolution Membrane Studies -- Correlative Fluorescence and EFTEM Imaging of the Organized Components of the Mammalian Nucleus -- High Data Output Method for 3-D Correlative Light-Electron Microscopy Using Ultrathin Cryosections -- Correlative Optical and Scanning Probe Microscopies for Mapping Interactions at Membranes -- Nanoimaging Cells Using Soft X-Ray Tomography -- Secondary Ion Mass Spectrometry Imaging of Biological Membranes at High Spatial Resolution.
520			_aFor more than a century, microscopy has been a centerpiece of extraordinary discoveries in biology.  Along the way, remarkable imaging tools have been developed allowing scientists to dissect the complexity of cellular processes at the nano length molecular scales.  Nanoimaging: Methods and Protocols presents a diverse collection of microscopy techniques and methodologies that provides guidance to successfully image cellular molecular complexes at nanometer spatial resolution.  The book's four parts cover: (1) light microscopy techniques with a special emphasis on methods that go beyond the classic diffraction-limited imaging; (2) electron microscopy techniques for high-resolution imaging of molecules, cells and tissues, in both two and three dimensions; (3) scanning probe microscopy techniques for imaging and probing macromolecular complexes and membrane surface topography;  and (4) complementary techniques on correlative microscopy, soft x-ray tomography and secondary ion mass spectrometry imaging.  Written in the successful format of the Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and accessible, Nanoimaging: Methods and Protocols highlights many of the most exciting possibilities in microscopy for the investigation of biological structures at the nano length molecular scales.
700	1		_aSousa, Alioscka A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aKruhlak, Michael J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627031363
776	0	8	_iPrinted edition: _z9781627031387
776	0	8	_iPrinted edition: _z9781493959617
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-137-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2013
999			_c235256 _d235256