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020 _a9781592590438
024 7 _a10.1385/1592590438
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aConnexin Methods and Protocols
_cedited by Roberto Bruzzone, Christian Giaume.
250 _a1st edition 2001
264 1 _aTotowa, NJ
_bHumana Press
_c2001
300 _a1 recurso en línea (XV, 491 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v154
505 0 _aTools to Study Connexins -- Investigation of Connexin Gene Expression Patterns by In Situ Hybridization Techniques -- Sodium Dodecyl Sulfate-Freeze-Fracture Immunolabeling of Gap Junctions -- Purification of Gap Junctions -- Culturing of Mammalian Cells Expressing Recombinant Connexins and Two-Dimensional Crystallization of the Isolated Gap Junctions -- Connexins/Connexons -- Biochemical Analysis of Connexon Assembly -- Expression and Imaging of Connexin-GFP Chimeras in Live Mammalian Cells -- Analysis of Connexin Expression in Brain Slices by Single-Cell Reverse Transcriptase Polymerase Chain Reaction -- Use of Retroviruses to Express Connexins -- Spatiotemporal Depletion of Connexins Using Antisense Oligonucleotides -- Transfection and Expression of Exogenous Connexins in Mammalian Cells -- Assays For Function -- Assaying the Molecular Permeability of Connexin Channels -- Applying the Xenopus Oocyte Expression System to the Analysis of Gap Junction Proteins -- Mutagenesis to Study Channel Structure -- Dual Patch Clamp -- Determining Ionic Permeabilities of Gap Junction Channels -- Fluorescence Recovery After Photobleaching -- Capture of Transjunctional Metabolites -- Physiology and Biology of Connexins -- The Study of Connexin Hemichannels (Connexons) in Xenopus Oocytes -- Exploring Hemichannel Permeability In Vitro -- Inducing De Novo Formation of Gap Juntion Channels -- Recording and Analysis of Putative Direct Electrical Interactions in the Mammalian Brain -- Intercellular Calcium Signaling and Flash Photolysis of Caged Compounds -- Biochemical Analysis of Connexin Phosphorylation -- How to Close a Gap Junction Channel.
520 _aAn essential element in direct cell-cell communication between virtually all cell types in vertebrates, connexins have been recently linked to an increasing number of human genetic diseases. In Connexin Methods and Protocols, well established investigators describe in detail their best techniques for studying the cellular and molecular biology of connexins, as well as their physiological properties. These forefront methods are specifically designed to investigate the life of connexins from biosynthesis to assembly into gap junction channels, and also include powerful assays to perform functional studies on these routes of cell-cell communication. To ensure experimental success, each time-tested technique contains detailed step-by-step instructions, as well as tips on how to avoid pitfalls. Also discussed are the many specific theoretical aspects of connexin biology and physiology. Cutting-edge and highly practical, Connexin Methods and Protocols offers laboratory researchers a comprehensive collection of proven methods for studying the role of connexins in intercellular communication and human genetic disorders, as well as finding promising new therapeutics for those disorders.
700 1 _aBruzzone, Roberto
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aGiaume, Christian
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617371196
776 0 8 _iPrinted edition:
_z9781489941961
776 0 8 _iPrinted edition:
_z9780896036581
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592590438
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2001
999 _c235259
_d235259