000			02959nam a22003615i 4500
001			235263
003			ES-VaUE
005			20221220020654.0
007			cr nn 008mamaa
008			100301s2009 xxu| s |||| 0|eng d
020			_a9781603275279
024	7		_a10.1007/978-1-60327-527-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aT Cell Protocols _cedited by Gennaro De Libero.
250			_a2nd edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XII, 166 páginas) _b41 ilustraciones, 3 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v514
505	0		_aAnalysis of Frequency and Phenotype of Antigen-Specific T Cells -- B Cell Helper Assays -- transkingdom RNA Interference (tkRNAi): A Novel Method to Induce Therapeutic Gene Silencing -- Flow Cytometry and Cell Activation -- Investigating T Cells by Polychromatic Flow Cytometry -- Generation of Human T Cell Clones -- Limiting Dilution Analysis of Antigen-Specific T Cells -- T Cell Epitope-Mapping by Cytokine Gene Expression Assay -- Cytokine Multiplex Immunoassay: Methodology and (Clinical) Applications -- Purification of the T Cell Antigen Receptor and Analysis by Blue-Native PAGE -- Non-Replicating Recombinant Vaccinia Virus Expressing CD80 to Enhance T-Cell Stimulation.
520			_aWith a wide variety of investigative approaches, T cell immunology is a vital and open field of study. In T Cell Protocols, Second Edition, an international panel of experts contribute fully updated classic protocols as well as newly established novel techniques for the study of T lymphocyte biology. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume provide brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections which collect expert tips on troubleshooting and avoiding known pitfalls. Up-to-date and easy to use, T Cell Protocols, Second Edition is an ideal guide for young investigators new to the complex field of immunology as well as a valuable, concise resource for experienced scientists searching for clear, efficacious descriptions of novel methods.
700	1		_aDe Libero, Gennaro _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781607611080
776	0	8	_iPrinted edition: _z9781617376610
776	0	8	_iPrinted edition: _z9781588295873
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-527-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2009
999			_c235263 _d235263