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001			235265
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008			121116s2013 xxu| s |||| 0|eng d
020			_a9781627031943
024	7		_a10.1007/978-1-62703-194-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aRice Protocols _cedited by Yinong Yang.
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XII, 335 páginas) _b43 ilustraciones, 7 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v956
505	0		_aRice Artificial Hybridization for Genetic Analysis -- Fluorescence In Situ Hybridization Techniques for Cytogenetic and Genomic Analyses -- Generation of Rice Mutants by Chemical Mutagenesis -- TILLING and Ecotilling for Rice -- Functional Characterization of Rice Genes Using a Gene-Indexed T-DNA Insertional Mutant Population -- QTL Analysis and Map-Based Cloning of Salt Tolerance Gene in Rice -- Site-Specific Gene Integration in Rice -- Transcriptome Profiling Analysis Using Rice Oligonucleotide Microarrays -- Cloning of Small RNAs for the Discovery of Novel MicroRNAs in Rice -- Global Identification of Small RNA Targets in Rice by Sequencing Sliced Ends of Messenger RNAs -- Artificial MicroRNAs for Specific Gene Silencing in Rice -- Rice Proteomic Analysis: Sample Preparation to Protein Identification -- Quantification of Jasmonic and Salicylic Acids in Rice Seedling Leaves -- Analysis of Insect-Induced Volatiles from Rice -- Phenotypic and Physiological Evaluation for Drought and Salinity Stress Responses in Rice -- Phenotypic, Physiological, and Molecular Evaluation of Rice Chilling Stress Response at the Vegetative Stage -- Analysis of Rice Root Hair Morphology Using Cryo-Scanning Electron Microscopy -- Inoculation and Virulence Assay for Bacterial Blight and Bacterial Leaf Streak of Rice -- Inoculation and Scoring Methods for Rice Sheath Blight Disease -- Molecular Approaches to Improve Rice Abiotic Stress Tolerance -- Molecular Strategies to Improve Rice Disease Resistance -- Molecular Strategies to Engineer Transgenic Rice Seed Compartments for Large-Scale Production of Plant-Made Pharmaceuticals -- Appendix: Genomic and Bioinformatic Resources for Rice Research.
520			_aWith the completion of a finished rice genome sequence, increasing efforts have focused on functional characterization of rice genes, elucidation of the underlying mechanisms involved in major agronomic traits (e.g., high yield, grain quality, abiotic stress tolerance, and disease resistance), and the subsequent translation of genomic knowledge into agricultural productivity via molecular breeding and improved cultural practice. To meet increasing interest in this field, Rice Protocols has been compiled to provide a series of core techniques and approaches commonly used in studying rice molecular biology and functional genomics. These approaches include genetic and molecular techniques such as artificial hybridization, fluorescence in situ hybridization, generation and characterization of chemical and T-DNA insertional mutants, quantitative trait loci (QTLs) analysis and map-based cloning, site-specific transgene integration, and artificial microRNA-mediated gene silencing, along with a variety of "omics" techniques.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and easy to use, Rice Protocols will prove useful for both beginners and experienced researchers whether they are molecular biologists who want to study rice plants or rice researchers who are interested in learning molecular techniques.
700	1		_aYang, Yinong _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627031936
776	0	8	_iPrinted edition: _z9781627031950
776	0	8	_iPrinted edition: _z9781493957644
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-194-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2013
999			_c235265 _d235265