000			04839nam a22003495i 4500
001			235271
003			ES-VaUE
005			20221220020655.0
007			cr nn 008mamaa
008			100301s1993 xxu| s |||| 0|eng d
020			_a9781592595082
024	7		_a10.1385/0896032396 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
100	1		_aHyde, John E. _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut
245	1	0	_aProtocols in Molecular Parasitology _cby John E. Hyde.
250			_a1st edition 1993
264		1	_aTotowa, NJ _bHumana Press _c1993
300			_a1 recurso en línea (XIV, 470 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v21
505	0		_aCulturing and Biological Cloning of Trypanosoma brucei -- Culturing and Biological Cloning of Trypanosoma cruzi -- In Vitro Cultivation and Biological Cloning of Leishmania -- Simple In Vitro Cultivation of the Malaria Parasite Plasmodium falciparum (Erythrocytic Stages) -- Synchronization and Cloning of Malaria Parasites -- The Culture and Preparation of Gametocytes of Plasmodium falciparum for Immunochemical, Molecular, and Mosquito Infectivity Studies -- The Culture of Schistosoma mansoni and Production of Life Cycle Stages -- Preparation of DNA and RNA from Trypanosoma brucei -- Isolation of RNA and DNA from Trypanosoma cruzi -- Isolation of DNA and RNA from Leishmania -- The Extraction and Purification of DNA and RNA from In Vitro Cultures of the Malaria Parasite Plasmodium falciparum -- Extraction and Purification of Nucleic Acids from Schistosomes -- Application of DNA and Whole Organisms to Filter Supports for DNA Probe Analysis: Dot, Slot, and Touch Blotting -- The Development and Use of Repetitive Sequences as DNA Probes for Parasite Detection and Species Identification -- Analysis of the DNA of Parasitic Protozoa by Flow Cytometry -- PCR orLowLevel Detection of Malaria Parasites in Blood -- PCR Methods for Identification of Point Mutations and Gene Rearrangements -- Antigenic Typing of Field Isolates of Plasmodium by DNA Techniques -- Ribosomal RNA Probes for Detection and Identification of Species -- Rapid Sequencing of Parasite rRNA -- Antibody Screening of Expression Libraries -- Antibody Select Procedure for Characterization of Expression Clones -- Isolation of Parasite Genes Using Synthetic Oligonucleotides -- Chromosome Mapping Methods for Parasitic Protozoa -- Transfection ofLeishmania and Trypanosoma brucei by Electroporation -- Two-Dimensional Polyacrylamide Gel Electrophoresis -- Isoenzyme Electrophoresis for Parasite Characterization -- The Surface Labeling of Schistosomes -- Monoclonal Antibody Affinity Chromatography -- Immunoblotting and Enzyme-Linked Immunosorbent Assay -- Immunofluorescence of Parasites -- Immunoelectron Microscopic Localization of Antigens in Malaria Parasites -- Preparation of Blotted Membrane for Protein Microsequencing -- Identification of T-Cell Epitopes.
520			_aProtocols in Molecular Parasitology offers a wide range of experi­ mental protocols, each written by experts, to research workers interested in exploring the molecular aspects of parasitology. This is a field that has expanded enormously in scope and potential over the last decade, although it is as yet significantly underrepresented in the literature out­ side of academic journals. The need for manuals such as this one stems in part from the increasing difficulty of reproducing experimental tech­ niques from reports in standard journal papers, where pressures on space usually result in highly abbreviated descriptions. The "Notes" sections of the following chapters are particularly aimed at counteracting this problem. Since the emphasis of the book is on aspects of molecular analysis, it was possible to devote only a limited amount of space to describing protocols for culture and isolation of the primary parasitic material. Therefore, of the numerous parasitic systems that are currently being investigated in laboratories around the world, only techniques for c- turing the "big five" are described here, since they collectively account for the great bulk of research in this field. It is also possible that some minor front-end modifications to certain protocols may be necessary, depending on exactly which parasite is being studied.
776	0	8	_iPrinted edition: _z9780896032392
776	0	8	_iPrinted edition: _z9781489940179
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896032396 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1993
999			_c235271 _d235271