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008			170512s2017 xxu| s |||| 0|eng d
020			_a9781493970308
024	7		_a10.1007/978-1-4939-7030-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	4	_aThe Renin-Angiotensin-Aldosterone System _bMethods and Protocols _cedited by Sean E. Thatcher.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (X, 201 páginas) _b58 ilustraciones, 38 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1614
505	0		_aA Brief Introduction into the Renin-Angiotensin-Aldosterone System: New and Old Techniques -- A Color Segmentation Based Method to Quantify Atherosclerotic Lesion Compositions with Immunostaining -- Assessment of Protein Carbonylation and Protein Tyrosine Phosphatase (PTP) Oxidation in Vascular Smooth Muscle Cells (VSMCs) using Immunoblotting Approaches -- Methods for Studying the Role of RAAS in the Modulation of Vascular Repair-Relevant Functions of Stem/Progenitor Cells -- Use of a Fluorescent Substrate to Measure ACE2 Activity in the Mouse Abdominal Aorta -- Measuring Blood Pressure using a Non-Invasive Tail Cuff Method in Mice -- Blood Pressure Monitoring using Radio Telemetry Method in Mice -- Characterization and Functional Phenotyping of Renal Immune Cells via Flow Cytometry -- Assessment of the Renin-Angiotensin System in Cellular Organelle: New Arenas for Study in the Mitochondria -- Comprehensive Assessments of Energy Balance in Mice -- In Vitro Assays to Determine Smooth Muscle Cell Hypertrophy, Protein Content, and Fibrosis -- A New Mouse Model for Introduction of Aortic Aneurysm by Implantation of Deoxycorticosterone Acetate Pellets or Aldosterone Infusion in the Presence of High Salt -- Fluorescence-Based Binding Assay for Screening Ligands of Angiotensin Receptors -- A Primer to Angiotensin Peptide Isolation, Stability, and Analysis by Nano-Liquid Chromatography with Mass Detection -- Analysis of Angiotensin Metabolism in the Kidney using Mass Spectrometry.
520			_aThis volume discusses protocols that aid in measuring different components in the renin-angiotensin-aldosterone system (RAAS). The book also looks at the methods used to assess angiotensin peptides and discerning the influence of RAAS components on different mammalian diseases. The chapters cover topics such as the use of fluorescent substrate to measure ACE2 activity in the mouse abdominal aorta, blood pressure monitoring using the radio telemetry method, and the analysis of angiotensin metabolism in the kidney using mass spectrometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and comprehensive, The Renin-Angiotensin-Aldosterone Systems: Methods and Protocols is a valuable resource that provides scientists and researchers with the best approaches to examine RAAS.
700	1		_aThatcher, Sean E _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493970285
776	0	8	_iPrinted edition: _z9781493970292
776	0	8	_iPrinted edition: _z9781493983735
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7030-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c235278 _d235278