000			03747nam a22003735i 4500
001			235283
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007			cr nn 008mamaa
008			121116s2013 xxu| s |||| 0|eng d
020			_a9781627032360
024	7		_a10.1007/978-1-62703-236-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_ap53 Protocols _cedited by Sumitra Deb, Swati Palit Deb.
250			_a2nd edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (X, 238 páginas) _b51 ilustraciones, 33 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v962
505	0		_aDetecting and Quantifying p53 Isoforms at mRNA Level in Cell Lines and Tissues -- Detecting p53 Isoforms at Protein Level -- Autophagy, Senescence and Apoptosis -- p53 and Cell Cycle Effects after DNA Damage -- p53 Ubiquitination and Proteasomal Degradation.-Identification of p53 in Mitochondria.-Identification of Novel Mutant p53 Interacting Proteins by Proteomic Analysis.-Identification of Small Molecules Affecting p53-MDM2/MDMX Interaction by Fluorescence Polarization -- Determine the Effect of p53 on Chemosensitivity.-Measurement of Chemosensitivity and Growth Rate in p53 Expressing Cells -- Mutant p53 in Cell Adhesion and Motility -- Use of the DNA Fiber Spreading Technique to Detect the  Effects of Mutant p53 on DNA Replication -- Generation of p53-deficient Induced Pluripotent Stem Cells (iPS) from Mouse Embryo Fibroblasts -- p53 Actions on MicroRNA Expression and Maturation Pathway -- Isolation and Characterization of Murine Multipotent Lung Stem Cells -- Generation of p53 Knock-down Cell Lines.-ChIP for Identification of p53 Responsive DNA Promoters -- ChIP-on-chip to Identify Mutant p53 Targets -- ChIP Sequencing to Identify p53 Targets -- The p53-Mdm2 Loop: A Critical Juncture of Stress Response.
520			_aThe second edition of p53 Protocols expands upon the first edition with progress in p53 research since the publication of the first edition. In p53 Protocols, Second Edition expert researchers in the field detail many of the methods that address the challenging questions of the p53 field.  These methods include the identification of the target genes and binding partners of gain of function p53 mutants, methods to determine stress response, autophagy or senescence induced by p53, cell cycle analysis, analysis of different phases of DNA replication modified by p53, and generation of pluripotent stem cells.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, p53 Protocols, Second Edition seeks to aid scientists in the further study into p53 and other tumor suppressors.
700	1		_aDeb, Sumitra _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aDeb, Swati Palit _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627032353
776	0	8	_iPrinted edition: _z9781627032377
776	0	8	_iPrinted edition: _z9781493959334
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-236-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2013
999			_c235283 _d235283