000			03854nam a22003735i 4500
001			235295
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007			cr nn 008mamaa
008			170317s2017 xxu| s |||| 0|eng d
020			_a9781493968909
024	7		_a10.1007/978-1-4939-6890-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aTechniques to Investigate Mitochondrial Function in Neurons _cedited by Stefan Strack, Yuriy M. Usachev.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XV, 344 páginas) _b74 ilustraciones, 51 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aNeuromethods _x1940-6045 _v123
505	0		_aThree-Dimensional Reconstruction of Neuronal Mitochondria by Electron Tomography -- Measuring Mitochondrial Shape with ImageJ -- Live Imaging Mitochondrial Transport in Neurons -- Techniques to Investigate Bioenergetics of Mitochondria -- Respirometry in Neurons -- Measuring ATP in Axons with FRET -- Characterizing Metabolic States using Fluorescence Lifetime Imaging Microscopy (FLIM) of NAD(P)H -- Techniques for Simultaneous Mitochondrial and Cytosolic Ca2+ Imaging in Neurons -- Live Imaging of Mitochondrial ROS Production and Dynamic Redox Balance in Neurons -- Monitoring of Permeability Transition Pore Openings in Isolated Individual Brain Mitochondria -- Examination of Mitochondrial Ion Conductance by Patch Clamp in Intact Neurons and Mitochondrial Membrane Preparations -- Monitoring of Permeability Transition Pore Openings in Mitochondria of Cultured Neurons -- Protocols for Assessing Mitophagy in Neuronal Cell Lines and Primary Neurons -- Examining Mitochondrial Function at Synapses In Situ -- Proteomic Analysis of Neuronal Mitochondria -- Measuring Mitochondrial Pyruvate Oxidation.
520			_aThis volume describes a broad spectrum of experimental approaches for investigating structure, function, and transport of neuronal mitochondria in health and disease. Most of these approaches were only recently developed and range from electron tomography-based 3D reconstruction of mitochondrial cristae to patch clamp recording from mitochondria in intact neurons. The chapters in this book cover topics such as mitochondrial proteomics, fluorescence lifetime imaging, respirometry and mitophagy, as well as optical approaches based on the use of genetically engineered fluorescent sensors for monitoring synaptic ATP and axonal ROS generation, mitochondrial Ca2+ cycling and pH changes, and mitochondrial dynamics and axonal trafficking in live neurons. Each chapter also discusses difficulties, tips, tricks, and precautions to take. Neuromethods series style chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Techniques to Investigate Mitochondrial Function in Neurons is a valuable and useful resource for a broad range of investigators interested in the function of neuronal mitochondria in health and disease states. .
700	1		_aStrack, Stefan _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aUsachev, Yuriy M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493968886
776	0	8	_iPrinted edition: _z9781493968893
776	0	8	_iPrinted edition: _z9781493983292
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6890-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c235295 _d235295