000			04283nam a22003855i 4500
001			235298
003			ES-VaUE
005			20221220020656.0
007			cr nn 008mamaa
008			170210s2017 xxu| s |||| 0|eng d
020			_a9781493967476
024	7		_a10.1007/978-1-4939-6747-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aProteomics _bMethods and Protocols _cedited by Lucio Comai, Jonathan E. Katz, Parag Mallick.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XII, 371 páginas) _b64 ilustraciones, 48 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1550
505	0		_aA Robust Protocol for Protein Extraction and Digestion -- Improving Proteome Coverage and Sample Recovery with Enhanced FASP (Efasp) For Quantitative Proteomic Experiments -- Proteome Characterization of a Chromatin Locus using the Proteomics of Isolated Chromatin Segments Approach -- Profiling Cell Lines Nuclear Sub-Proteome -- Optimized Enrichment of Phosphoproteomes by Fe-IMAC Column Chromatography -- Full Membrane Protein Coverage Digestion And Quantitative Bottom-Up Mass Spectrometry Proteomics -- Hydrophilic Strong Anion Exchange (Hsax) Chromatography Enables Deep Fractionation Of Tissue Proteomes -- High Ph Reversed-Phase Micro-Columns for Simple, Sensitive and Efficient Fractionation of Proteome and (TMT-Labeled) Phosphoproteome Digests -- Multi-Lectin Affinity Chromatography for Separation, Identification, and Quantitation of Intact Protein Glycoforms in Complex Biological Mixtures -- Parallel Exploration of Interaction Space by Bioid and Affinity Purification Coupled to Mass Spectrometry -- LUMIER: A Discovery Tool for Mammalian Protein Interaction Networks -- Dual Color, Multiplex Analysis of Protein Microarrays for Precision Medicine -- Quantitative Proteomics Using SILAC -- Relative Protein Quantification using Tandem Mass Tag Mass Spectrometry -- Pathway-Informed Discovery and Targeted Proteomic Workflows Using Mass Spectrometry -- Generation of High Quality SWATH Acquisition Data for Label-Free Quantitative Proteomics Studies Using Tripletof® Mass Spectrometers -- Annotating Mutational Effects on Proteins and Protein Interactions: Designing Novel and Revisiting Existing Protocols -- Protein Micropatterning Assay - Quantitative Analysis of Protein-Protein Interactions -- Designing Successful Proteomics Experiments -- Automated SWATH Data Analysis using Targeted Extraction of Ion Chromatograms -- Virtualization of Legacy Instrumentation Control Computers for Improved Reliability, Operational Life, and Management -- Statistical Assessment of QC Metrics on Raw LC-MS/MS Data -- Data Conversion with proteoWizard msConvert.
520			_aThis volume aims to provide protocols on a wide range of biochemical methods, analytical approaches, and bioinformatics tools developed to analyze the proteome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Proteomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.
700	1		_aComai, Lucio _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aKatz, Jonathan E _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aMallick, Parag _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493967452
776	0	8	_iPrinted edition: _z9781493967469
776	0	8	_iPrinted edition: _z9781493982905
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6747-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c235298 _d235298