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008			150820s2016 xxu| s |||| 0|eng d
020			_a9781493928095
024	7		_a10.1007/978-1-4939-2809-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aT-Cell Development _bMethods and Protocols _cedited by Rémy Bosselut, Melanie S. Vacchio.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XI, 297 páginas) _b52 ilustraciones, 24 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1323
505	0		_a200 Million Thymocytes and I: A Beginner's Survival Guide to T cell Development -- Development of gd T Cells, the Special Forces Soldiers of the Immune System -- Genetic Tools to Study T Cell Development -- Assessment of T cell Development by Flow Cytometry -- Flow Cytometry Analysis of Thymic Cells and their Subpopulations -- Identifying the Spatial Relationships of Thymic Stromal and Thymocyte Subsets by Immunofluorescence Analysis -- Purification of Thymocyte and T Cell Subsets -- Retroviral Transduction of T Cells and T Cell Precursors -- Bone Marrow and Fetal Liver Radiation Chimeras -- In vitro Analyses of T Cell Effector Differentiation -- Studying T Cell Development in Thymic Slices -- FTOC-based Analysis of Negative Selection -- Reconstituted Thymus Organ Culture -- Induction of T-cell Development in vitro by Delta-like (Dll)-Expressing Stromal Cells -- In vitro Analysis of Thymocyte Signaling -- Molecular Analysis of Mouse T Cell Receptor a and b Gene Rearrangements -- Intrathymic Injection -- Analysis of Cell Proliferation and |Homeostasis using EdU Labeling -- Characterization and Isolation of Human T Cell Progenitors -- Approaches to Study Human T Cell Development -- Humanized Mice to Study Human T Cell Development -- Using the Zebrafish Model to Study T cell Development.
520			_aThis volume provides simple and accessible experiment protocols to explore thymus biology. T-Cell Development: Methods and Protocols is divided into three parts presenting short reviews on T cell development, analysis strategies, protocols for cell preparation, flow cytometry analyses, and multiple aspects of thymocyte biology. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.  Concise and easy-to-use, T-Cell Development: Methods and Protocols aims to ensure successful results in the further study of this vital field.
700	1		_aBosselut, Rémy _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aS. Vacchio, Melanie _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493928088
776	0	8	_iPrinted edition: _z9781493928101
776	0	8	_iPrinted edition: _z9781493948437
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2809-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c235304 _d235304