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020 _a9781592596553
024 7 _a10.1385/159259655X
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aProtein Purification Protocols
_cedited by Paul Cutler.
250 _a2nd edition 2004
264 1 _aTotowa, NJ
_bHumana Press
_c2004
300 _a1 recurso en línea (XII, 484 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v244
505 0 _aGeneral Strategies -- Preparation of Extracts From Animal Tissues -- Protein Extraction From Plant Tissues -- Extraction of Recombinant Protein From Bacteria -- Protein Extraction From Fungi -- Subcellular Fractionation of Animal Tissues -- Subcellular Fractionation of Plant Tissues -- The Extraction of Enzymes From Plant Tissues Rich in Phenolic Compounds -- Avoidance of Proteolysis in Extracts -- Concentration of Extracts -- Making and Changing Buffers -- Purification and Concentration by Ultrafiltration -- Bulk Purification by Fractional precipitation -- Ion-Exchange Chromatography -- Hydrophobic Interaction Chromatography -- Affinity Chromatography -- Dye-Ligand Affinity Chromatography -- Lectin Affinity Chromatography -- Immunoaffinity Chromatography -- Immobilized Metal-Ion Affinity Chromatography -- Chromatography on Hydroxyapatite -- Thiophilic Affinity Chromatography and Related Methods -- Affinity Precipitation Methods -- Isoelectric Focusing -- Chromatofocusing -- Size-Exclusion Chromatography -- Fast Protein Liquid Chromatography -- Reversed-Phase Chromatography of Proteins -- Extraction of Membrane Proteins -- Removal of Detergent From Protein Fractions -- Purification of Membrane Proteins -- Lyophilization of Proteins -- Storage of Pure Proteins -- Electroelution of Proteins From Polyacrylamide Gels -- Electroblotting of Proteins From Polyacrylamide Gels -- Two-Dimensional Polyarcylamide Gel Electrophoresis for Proteome Analyses -- Microscale Solution Isoelectrofocusing -- Practical Column Chromatography -- Detection Methods -- Peptide Proteomics -- Multidimensional Liquid Chromatography of Proteins -- Mass Spectrometry -- Purification of Therapeutic Proteins -- Purification Process Scale-Up.
520 _aIn this new edition of the very successful Protein Purification Protocols (1996), Paul Cutler completely updates the existing protocols to reflect recent advances and adds an enormous new array of proteomic techniques for protein isolation and analysis. These cutting-edge techniques include not only two-dimensional gel electrophoresis for analysis and characterization, but also analytical chromatography for multidimensional separations of proteins and peptides, and mass spectrometry for isolating proteins. With the many recent advances in technology, simple spectrometric detection is no longer the only option for separating proteins, and the authors treat in full detail all the newer methods for these separations. Comprehensive and highly practical, Protein Purification Protocols, Second Edition, brings together all the key methodologies that both novice and experienced investigators need to carry out successful experimental work on proteins and their functions today.
700 1 _aCutler, Paul
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617373343
776 0 8 _iPrinted edition:
_z9781489939432
776 0 8 _iPrinted edition:
_z9781588290670
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/159259655X
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2004
999 _c235305
_d235305