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008			100301s2000 xxu| s |||| 0|eng d
020			_a9781592590421
024	7		_a10.1385/159259042X _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aNeuropeptide Y Protocols _cedited by Ambikaipakan Balasubramaniam.
250			_a1st edition 2000
264		1	_aTotowa, NJ _bHumana Press _c2000
300			_a1 recurso en línea (X, 246 páginas) _b52 ilustraciones, 3 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v153
505	0		_aCloning Techniques -- Cloning Neuropeptide Tyrosine cDNA -- Human Y1/Y5 Receptor Gene Cluster -- Human Type 2 Neuropeptide Y Receptor Gene -- Y4 Receptor in Different Species -- NeuropeptideYY5 Receptor Expression -- Homology-Based Cloning Methods -- Production of Transgenic and Knock-Out Models -- Developing Transgenic NeuropeptideY Rats -- NeuropeptideYY1 Receptor-Deficient Mice -- Antisense Technology -- Antisense Oligonucleotide Approach to Study NPY-Mediated Feeding Signal Transduction -- A G?i RNA-Antisense Expression Strategy to Investigate Coupling of Peptide YY/Neuropeptide Y Receptor to G?i -- NPY Y5 Receptor Subtype -- NPY Antisense Oligodeoxynucleotides to Study the Actions of NPY -- Techniques to Study Tissue Distribution of mRNA -- Localization of Y-Receptor Subtype mRNAs in Rat Brain by Digoxigenin Labeled In Situ Hybridization -- Central Y4 Receptor Distribution -- Combining Non-Isotopic Localization of NPY mRNA with Immunocytochemistry -- Miscellaneous Techniques -- Y Receptors Characterized by RT-PCR -- Quantification of NPY mRNA by Ribonuclease Protection Assay -- Radioligand Binding Studies.
520			_aIt is now clear that neuropeptide Y (NPY), the most abundant peptide present in the mammalian nervous system, is implicated in the pathophysiology of many diseases, including eating disorders, seizures, memory loss, anxiety, depression, and cardiovascular diseases, and offers major therapeutic opportunities for their treatment. In Neuropeptide Y Protocols, a strong cohort of established investigators from a variety of disciplines detail their best techniques for advancing NPY research. Among the many readily reproducible methods described are those for cloning, for the production of transgenic and knockout models, and for the use of antisense technology to define the receptors and the signal transduction pathways mediating NPY actions. A range of the major qualitative and quantitative techniques used to study tissue mRNA distribution are also included, as well as methods for radioligand binding. The cloning techniques utilize genomic DNA isolation, expression cloning, PCR cloning, the construction of hybrid receptors, and homology-based cloning. Highly practical and state-of-the-art, Neuropeptide Y Protocols constitutes a gold-standard collection of proven techniques for advancing today's understanding of this significant neuropeptide, as well as for developing the promising NPY-based therapeutics now emerging for many important disorders.
700	1		_aBalasubramaniam, Ambikaipakan _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489941527
776	0	8	_iPrinted edition: _z9780896036628
776	0	8	_iPrinted edition: _z9781489941510
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/159259042X _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2000
999			_c235309 _d235309