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008 111205s2012 xxu| s |||| 0|eng d
020 _a9781617794278
024 7 _a10.1007/978-1-61779-427-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aNext-Generation MicroRNA Expression Profiling Technology
_bMethods and Protocols
_cedited by Jian-Bing Fan.
250 _a1st edition 2012
264 1 _aTotowa, NJ
_bHumana Press
_c2012
300 _a1 recurso en línea (XI, 341 páginas)
_b64 ilustraciones, 11 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v822
505 0 _aControlling miRNA Regulation in Disease -- Introduction to miRNA Profiling Technologies and Cross-Platform Comparison -- Stem-Loop RT-qPCR for MicroRNA Expression Profiling -- Poly(T) Adaptor RT-PCR -- MicroRNA In Situ Hybridization -- Agilent MicroRNA Microarray Profiling System -- miRNA Gene Expression Profiling Using Illumina Universal BeadChips -- MicroRNA Expression Analysis Using the Affymetrix Platform -- Individualized miRNA Assay Panels Using Optically Encoded Beads -- Microfluidic Primer Extension Assay (MPEA) -- MicroRNA Profiling Using µParaflo Microfluidic Array Technology -- MicroRNA Expression Analysis Using the Illumina MicroRNA-Seq Platform -- Next Generation Sequencing of miRNAs with Roche 454 GS-FLX Technology: Steps for a Successful Application -- Methods for Small RNA Preparation for Digital Gene Expression Profiling by Next-Generation Sequencing -- Profiling of Short RNAs Using Helicos Single-Molecule Sequencing -- deepBase: Annotation and Discovery of microRNAs and Other Non-Coding RNAs from Deep Sequencing Data -- PhenomiR: MicroRNAs in Human Diseases and Biological Processes -- miRNA Expression Profiling: From Reference Genes to Global Mean Normalization -- miRNA Data Analysis: Next-Gen Sequencing -- Integrated miRNA Expression Analysis and Target Prediction -- miRNAs in Human Cancer -- Blood-Based miRNA Preparation for Non-Invasive Biomarker Development.
520 _aThe rapid pace of microRNA (miRNA) research continues to drive the advances of techniques for miRNA expression profiling, and innovative technologies that are more sensitive, specific, quantitative, and that are compatible with a wide range of biospecimens have been developed during the past few years.  In Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols, expert researchers in the field contribute detailed examinations of the most current approaches being used today.  This volume includes comprehensive coverage of methodologies that have been developed for miRNA profiling, as well as next-gen sequencing technology, miRNA databases, and specialized applications, such as cancer studies and miRNA-based non-invasive biomarker development.  Written in the highly successful Methods in Molecular Biology™ series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols provides an ideal guide for novices and experts alike who are seeking to utilize these powerful technologies.
700 1 _aFan, Jian-Bing
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617794261
776 0 8 _iPrinted edition:
_z9781617794285
776 0 8 _iPrinted edition:
_z9781493962174
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-427-8
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2012
999 _c235317
_d235317