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020 _a9781627033459
024 7 _a10.1007/978-1-62703-345-9
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aChemical Neurobiology
_bMethods and Protocols
_cedited by Matthew R. Banghart.
250 _a1st edition 2013
264 1 _aTotowa, NJ
_bHumana Press
_c2013
300 _a1 recurso en línea (X, 202 páginas)
_b70 ilustraciones, 1 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v995
505 0 _aEngineering K+ Channels using Semisynthesis -- Chemical Derivatization and Purification of Peptide-Toxins for Probing Ion Channel Complexes -- Using Yeast to Study Potassium Channel Function and Interactions with Small Molecules -- A FLIPR Assay for Evaluating Agonists and Antagonists of GPCR Heterodimers -- Characterizing Caged Molecules Through Flash Photolysis and Transient Absorption Spectroscopy -- Characterization of One- and Two- Photon Photochemical Uncaging Efficiency -- Photochromic Potassium Channel Blockers: Design and Electrophysiological Characterization -- A 1H NMR Assay for Measuring the Photostationary States of Photoswtichable Ligands -- Developing a Photoreactive Antagonist -- Development and in vitro Characterization of Ratiometric and Intensity-based Fluorescent Ion Sensors.-  Characterization of Voltage-Sensitive Dyes in Living Cells using Two-Photon Excitation -- Characterization and Validation of Fluorescent Receptor Ligands: A Case Study of the Ionotropic Serotonin Receptor.-  Imaging Single Synaptic Vesicles in Mammalian Central Synapses with Quantum Dots.-  Directed Evolution of Protein-Based Neurotransmitter Sensors for MRI.
520 _aMany advances in modern neuroscience are enabled by the availability of chemical tools that allow sensitive, precise, and quantitative measurements of, and control over, biological processes. These powerful reagents are widely used for investigating the nervous system at levels of detail ranging from ion channel structure to neural network dynamics. Recent advances in photochemistry, microscopy, and protein engineering have triggered a surge in the development and application of these interdisciplinary techniques. Chemical Neurobiology: Methods and Protocols assists with the design, characterization and validation of new chemical tools for neurobiology by providing detailed protocols of procedures and assays deemed essential for the successful development and implementation of such tools. Divided into three sections, topics cover chemical probes of membrane protein structure and function, photochemical control of protein and cellular function, and chemical probes for imaging in the nervous system. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Chemical Neurobiology: Methods and Protocols serves scientists at many levels, including students aiming to expand their perspective, laboratory researchers seeking technical guidance, and established investigators looking for creative solutions to their research problems in molecular, cellular and systems neuroscience. .
700 1 _aBanghart, Matthew R
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781627033442
776 0 8 _iPrinted edition:
_z9781627033466
776 0 8 _iPrinted edition:
_z9781493957828
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-345-9
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2013
999 _c235321
_d235321