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020 _a9781627035620
024 7 _a10.1007/978-1-62703-562-0
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aPhosphatase Modulators
_cedited by José Luis Millán.
250 _a1st edition 2013
264 1 _aTotowa, NJ
_bHumana Press
_c2013
300 _a1 recurso en línea (X, 315 páginas)
_b75 ilustraciones, 45 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1053
505 0 _aChemical Probe Development Versus Drug Development -- Phosphatase High-Throughput Screening Assay Design and Selection -- Multisystemic Functions of Alkaline Phosphatases -- Robotic Implementation of Assays: Tissue-Nonspecific Alkaline Phosphatase (TNAP) Case Study -- Inhibitors of Tissue-Nonspecific Alkaline Phosphatase (TNAP): From Hits to Leads -- A Method for Direct Assessment of Tissue-Nonspecific Alkaline Phosphatase (TNAP) Inhibitors in Blood Samples -- Isolation and Characteristics of Matrix Vesicles -- The Use of Tissue-Nonspecific Alkaline Phosphatase (TNAP) and PHOSPHO1 Inhibitors to Affect Mineralization by Cultured Cells -- Modulators of Intestinal Alkaline Phosphatase -- New Activity Assays for ENPP1 with Physiological Substrates ATP and ADP -- Structure of Acid Phosphatases -- Purification of Prostatic Acid Phosphatase (PAP) for Structural and Functional Studies -- Protein Tyrosine Phosphatases: Structure, Function, and Implication in Human Disease -- High-Throughput Screening for Protein Tyrosine Phosphatase Activity Modulators -- Evaluating Effects of Tyrosine Phosphatase Inhibitors on T Cell Receptor Signaling -- Interactor-Guided Dephosphorylation by Protein Phosphatase-1 -- Structure, Regulation, and Pharmacological Modulation of PP2A Phosphatases.
520 _aDriving further the research on mammalian alkaline phosphatase structure and function, Phosphatase Modulators collects expert contributions into one "how to" manual for basic scientists interested in initiating a drug discovery effort. While this book contains the traditional method chapters and some typical reviews on the structure and known functions of phosphatases, other contributions are meant to discuss approaches and alternatives useful in making "go/no-go" decisions in high throughput screening (HTS) and lead optimization campaigns. Many chapters focus on tissue-nonspecific alkaline phosphatase (TNAP) as well as protein phosphatases.  Written for the highly successful Methods in Molecular Biology series, chapters in this volume include the kind of detail and key implementation advice that promotes reproducible results.   Step-by-step and practical, Phosphatase Modulators offers a path to understanding many of the facets and complexities associated with undertaking a drug discovery effort and will serve as a roadmap to initiating those efforts.
700 1 _aMillán, José Luis
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781627035637
776 0 8 _iPrinted edition:
_z9781627035613
776 0 8 _iPrinted edition:
_z9781493960316
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-562-0
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2013
999 _c235324
_d235324