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020 _a9781592595334
024 7 _a10.1385/0896032973
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
100 1 _aHoward, Jenny.
_eautor
_4aut
_4http://id.loc.gov/vocabulary/relators/aut
245 1 0 _aDiagnostic Bacteriology Protocols
_cby Jenny Howard, David M. Whitcombe.
250 _a1st edition 1995
264 1 _aTotowa, NJ
_bHumana Press
_c1995
300 _a1 recurso en línea (X, 283 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v46
505 0 _aAntibiotic Resistance Profiling -- Bacteriocin Typing -- Bacteriophage Typing -- The Analysis of Bacterial Proteins by SDS Polyacrylamide Gel Electrophoresis -- Lipopolysaccharide Chemotyping -- Analysis of Bacterial Outer Membrane Proteins -- Multilocus Enzyme Electrophoresis -- Fast Atom Bombardment-Mass Spectrometry -- Pyrolysis Mass Spectrometry -- The Direct Immunofluorescent Filter Technique (DIFT) -- The Direct Epifluorescent Filter Technique (DEFT) -- Use of Commercially Available ELISA Kits for Detection of Foodborne Pathogens -- Detection of Foodborne Pathogens Using DNA Probes and a Dipstick Format -- Preparation of Bacterial Genomic DNA -- Pulsed Field Gel Electrophoresis -- Application of Total DNA Restriction Pattern Analysis to Identification and Differentiation of Bacterial Strains -- Determination of rRNA Gene Restriction Patterns -- Preparation of Nonradioactive DNA Probes -- Preparation of Bacterial Plasmid DNA -- Plasmid Profile Typing and Plasmid Fingerprinting -- Polymerase Chain Reaction or the Detection of Listeria Species and Listeria monocytogenes -- Development of Bacterial Species-Specific DNA Probes Based on Ribosomal RNA Genes Using PCR -- Identification of Microorganisms Using Random Primed PCR -- Ligase Chain Reaction.
520 _aEnhanced public awareness of microbiological issues and increased governmental regulation have ensured that microbiology and bacteriology remain important, from the academic laboratory to a broad range of indus­ trial environments. Since the techniques used in bacteriology vary widely and have advanced rapidly of late, it has become increasingly desirable to gather a collection of up-to-date protocols from a comprehensive range of disciplines into a single volume. Diagnostic Bacteriology Protocols thus aims to provide a wide variety of up-to-date protocols for the identification and differentiation of bacteria from pure or mixed cultures, and for the study of bacteria at the genus through to subspecies levels. In addition, we aim to provide methods covering a range of complexities, some requiring little or no special equipment, others exploiting recent advances in sophisticated instrumentation or novel nucleic acid-based technology. We also present techniques to study a variety of aspects of bacteria-from the features of whole cells and their growth, through their enzyme activities and structural components, to nucleic ac- based analysis.
700 1 _aWhitcombe, David M.
_eautor
_4aut
_4http://id.loc.gov/vocabulary/relators/aut
776 0 8 _iPrinted edition:
_z9781489940780
776 0 8 _iPrinted edition:
_z9780896032972
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896032973
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_1995
999 _c235326
_d235326