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001			235339
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007			cr nn 008mamaa
008			100301s2006 xxu| s |||| 0|eng d
020			_a9781592599684
024	7		_a10.1385/1592599680 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aDifferential Display Methods and Protocols _cedited by Peng Liang, Jonathan Meade, Arthur B. Pardee.
250			_a2nd edition 2006
264		1	_aTotowa, NJ _bHumana Press _c2006
300			_a1 recurso en línea (XIV, 320 páginas) _b132 ilustraciones, 1 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v317
505	0		_aMethodologies -- Global Analysis of Gene Expression by Differential Display -- Automation of Fluorescent Differential Display With Digital Readout -- Ordered Differential Display -- GeneCalling® -- High-Density Sampling Differential Display of Prokaryotic mRNAs With RAP-PCR -- Vertical Arrays -- Automated Pattern Ranking in Differential Display Data Analysis -- Linking cDNA-AFLP-Based Gene Expression Patterns and ESTs -- Applications -- Differentially Expressed Genes Associated With Hepatitis B Virus HBx and MHBs Protein Function in Hepatocellular Carcinoma -- Identification of Disease Markers by Differential Display -- Saturation Screening for p53 Target Genes by Digital Fluorescent Differential Display -- Identification of p53-Regulated Genes by the Method of Differential Display -- Identification by Differential Display of IL-24 Autocrine Loop Activated by Ras Oncogenes -- Comprehensive Analysis of Ovarian Gene Expression During Ovulation Using Differential Display -- Functional Analysis of Nocturnin -- Isolation and Characterization of Anthocyanin 5-O-Glucosyltransferase in Perilla frutescens var. crispa by Differential Display -- Identification of Target Genes of a Yeast Transcriptional Repressor -- Detection of an mRNA Polymorphism by Differential Display -- Silencing in Yeast -- Identification of mRNA Bound to RNA Binding Proteins by Differential Display.
520			_aCarrying on the high standards of the first edition of Differential Display Methods, Peng Liang et al. have based their second edition on a new mathematical model of differential display (DD) that takes advantage of automation, as well as digital data acquisition and analysis. These well-versed authors explain and highlight all the recent methodological refinements, including automated liquid handling of hundreds of DD PCR reaction setups combined with capillary electrophoresis, a prototype computer program to automatically allow positive band identification from a fluorescence differential display image, and restriction fragment-based DD screenings that can link any cDNA fragment directly to a given gene once the sequence information of all transcripts becomes available. Other improvements discussed are combining DD and DNA microarrays by reducing the complexity of cDNA probes while increasing the sensitivity of detection, and a DD approach to detect prokaryotic mRNA expression. The authors also demonstrate the power of DD technology with a collection of outstanding examples of DD applications and detailed experimental procedures. The elegant studies described here have led to the discovery of many important genes involved in viral infection, Prion disease, cancer, ovulation, circadian clock, floral color, transcription repression gene silencing, mRNA polymorphism, and protein-RNA interaction. State-of-the-art and highly practical, Differential Display Methods, Second Edition offers gene hunters the possibility of genome-wide comprehensive DD screening, as well as a proven road map for any successful "gene fishing" expedition.
700	1		_aLiang, Peng _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aMeade, Jonathan _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aPardee, Arthur B _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617375033
776	0	8	_iPrinted edition: _z9781588293381
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592599680 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2006
999			_c235339 _d235339