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020 _a9781493916252
024 7 _a10.1007/978-1-4939-1625-2
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aSalmonella
_bMethods and Protocols
_cedited by Heide Schatten, Abraham Eisenstark.
250 _a2nd edition 2015
264 1 _aNew York, NY
_bSpringer International Publishing
_c2015
300 _a1 recurso en línea (XI, 301 páginas)
_b52 ilustraciones, 26 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1225
505 0 _aLuminex® Multiplex Bead Suspension Arrays for the Detection and Serotyping of Salmonella spp. -- Quantitative Proteomic Identification of Host Factors Involved in the Salmonella typhimurium Infection Cycle -- Determination of Antimicrobial Resistance in Salmonella spp. -- Red-mediated Recombineering of Salmonella enterica Genomes -- A Method to Introduce an Internal Tag Sequence into a Salmonella Chromosomal Gene -- Generation and use of Site-directed Chromosomal cyaA' Translational Fusions in Salmonella enteric -- Detection of Antimicrobial (Poly)Peptides with Acid Urea Polyacrylamide Gel Electrophoresis followed by Western Immunoblot -- Detecting non-typhoid Salmonella in Humans by Enzyme-linked Immunosorbent Assays (ELISAs): Practical and Epidemiological Aspects -- Study of the Stn Protein in Salmonella; A Regulator of Membrane Composition and Integrity -- Development of a Bacterial Nanoparticle Vaccine -- Direct Attachment of Nanoparticle Cargo to Salmonella typhimurium Membranes Designed for Combination Bacteriotherapy Against Tumors -- Applications of Microscopy in Salmonella Research -- Live Cell Imaging of Intracellular Salmonella enteric -- In vitro Modeling of Gallbladder-associated Salmonella spp. Colonization -- Salmonella Phages and Prophages - Genomics, Taxonomy,  and Applied Aspects.
520 _aSalmonella: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed methods on different aspects and landmarks advancing knowledge on salmonella research. With new chapters on molecular assays for detection, identification and serotyping of salmonella, quantitative proteomic identification of host factors involved in salmonella infection, determination of antimicrobial resistance in salmonella, site-directed mutagenesis, chromosomal gene analysis, development of bacterial nanoparticle vaccine, attachment of nanoparticle cargo to biotinylated salmonella for combination bacteriotherapy against tumors, various microscopy methods to analyze salmonella interaction with host cells, in vitro modeling of gallbladder-associated salmonella colonization, and analysis of salmonella phages and prophages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Salmonella: Methods and Protocols, Second Edition present methods that are of value to investigators in the salmonella field.
700 1 _aSchatten, Heide
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aEisenstark, Abraham
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493916269
776 0 8 _iPrinted edition:
_z9781493916245
776 0 8 _iPrinted edition:
_z9781493954407
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1625-2
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2015
999 _c235340
_d235340