000			04681nam a22003615i 4500
001			235342
003			ES-VaUE
005			20221220020659.0
007			cr nn 008mamaa
008			140830s2014 xxu| s |||| 0|eng d
020			_a9781493914357
024	7		_a10.1007/978-1-4939-1435-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aStem Cells and Tissue Repair _bMethods and Protocols _cedited by Chrissa Kioussi.
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XII, 250 páginas) _b50 ilustraciones, 42 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1210
505	0		_aCulturing and Differentiating Mouse Embryonic Stem Cells -- Neural Stem Cells Transplantation in an Animal Model of Traumatic Brain Injury -- Experimental Cell Transplantation for Traumatic Spinal Cord Injury Regeneration: Intramedullar or Intrathecal Administration -- Generation of Murine Xenograft Models of Brain Tumors from Primary Human Tissue for in vivo Analysis of the Brain Tumor Initiating Cell -- Growth of Bone Marrow and Skeletal Muscle Side Population Stem Cells in Suspension Culture -- Isolation, Culture and Immunostaining of Skeletal Muscle Fibers to Study Myogenic Progression in Satellite Cells -- Human Neural Crest Stem Cells Derived from Human Pluripotent Stem Cells -- Dental Pulp Stem Cells (DPSC) Isolation, Characterization and Differentiation -- Dental Pulp Stem Cells Isolation and Osteogenic Differentiation: A Good Promise for Tissue Engineering -- Efficient Hepatic Differentiation of Human Induced Pluripotent Stem Cells in A Three-Dimensional Microscale Culture -- The Generation and Maintenance of Rat Induced Pluripotent Stem Cells -- Protocol for Cutaneous Wound Healing Assay in a Murine Model -- Adipose Derived Stem Cells: Methods for Isolation and Applications for Clinical Use -- In vitro Detection of Residual Undifferentiated Cells in Retinal Pigment Epithelial Cells Derived from Human Induced Pluripotent Stem Cells -- Whole Mount Immunohistochemistry to Study Spermatological Stem Cells and Spermatogenic Lineage in Mice, Monkeys and Humans -- Differentiating the Stem Cell Pool of Human Hair Follicle Outer Root Sheath into Functional Melanocytes -- Pancreas Development ex vivo: Culturing Embryonic Pancreas Explants on Permeable Culture Inserts, with Firbonectin-Coated Glass Microwells, or Embedded in Three-Dimensional MatrigelTM -- Ultra-Rapid Manufacturing of Engineered Epicardial Substitute to Regenerate Cardiac Tissue Following Acute Ischemic Injury.
520			_aStem Cells and Tissue Repair: Methods and Protocols presents in-depth methods for the three major approaches of rejuvenating an aging or sick body: latent regenerative capacity stimulated in a targeted way, replacement organs grown de novo and surgically implanted, and tissue surgically implanted and coaxed to integrate and restore problem areas. The first approach taps into the latent regenerative capacity of particular tissues, such as muscle, skin, fat, or bone marrow. The second approach induces and grows pluripotent stem cells, drives their differentiation along certain pathways such as germ layers, neural crest, liver, teeth or retina, and cultures organs such as pancreas and heart. While the third approach involves various engraftment techniques for neural tissue. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Stem Cells and Tissue Repair: Methods and Protocols provides state-of the-art methods descriptions and the references therein that will provide a suitable starting point for exploring the vast literature that has already developed for regenerative medicine.
700	1		_aKioussi, Chrissa _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493914364
776	0	8	_iPrinted edition: _z9781493914340
776	0	8	_iPrinted edition: _z9781493955329
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1435-7 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c235342 _d235342