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| 008 | 141127s2015 xxu| s |||| 0|eng d | ||
| 020 | _a9781493921522 | ||
| 024 | 7 |
_a10.1007/978-1-4939-2152-2 _2doi |
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| 040 |
_aES-MaUEC _bspa _cES-MaUEC |
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| 245 | 1 | 0 |
_aNeuronal Cell Death _bMethods and Protocols _cedited by Laura Lossi, Adalberto Merighi. |
| 250 | _a1st edition 2015 | ||
| 264 | 1 |
_aNew York, NY _bSpringer International Publishing _c2015 |
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| 300 |
_a1 recurso en línea (XIV, 396 páginas) _b82 ilustraciones, 48 ilustraciones a color |
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| 336 |
_atexto _btxt _2rdacontent |
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_aelectrónico _bc _2rdamedia |
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_arecurso electrónico _bcr _2rdacarrier |
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| 347 |
_aarchivo de texto _bPDF |
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_aMethods in Molecular Biology _x1940-6029 _v1254 |
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| 505 | 0 | _aNeuronal Cell Death: An Overview of its Different Forms in Central and Peripheral Neurons -- Cell Volume Regulation Monitored with Combined Epifluorescence and Digital Holographic Microscopy -- Flow Cytometric Analysis of DNA Synthesis and Apoptosis in Central Nervous System Using Fresh Cell Nuclei -- Nuclear Signs of Pre-Neurodegeneration -- Multi-Parametric O2 Imaging in Three-Dimensional Neural Cell Models with the Phosphorescent Probes -- Calcium Imaging in Neuron Cell Death -- Monitoring Mitochondrial Membranes Permeability in Live Neurons and Mitochondrial Swelling through Electron Microscopy Analysis -- Real-Time Visualization of Caspase-3 Activation by Fluorescence Resonance Energy Transfer (FRET) -- Design and Cloning of Short Hairpin RNAs (shRNAs) into a Lentiviral Silencing Vector to -- Genomic Analysis Using Affymetrix Standard Microarray GeneChips (169 Format) in Degenerate Murine Retina -- Genomic Analysis of Transcriptional Changes Underlying Neuronal Apoptosis -- High-Throughput Cell Death Assays -- Image Analysis Algorithms for Immunohistochemical Assessment of Cell Death -- In Vitro Oxygen-Glucose Deprivation to Study Ischemic Cell Death -- Laser Microbeam Targeting of Single Nerve Axons in Cell Culture -- Real-Time Imaging of Retinal Cell Apoptosis by Confocal Scanning Laser Ophthalmoscopy -- Targeted Toxicants to Dopaminergic Neuronal Cell Death -- Stem Cells, Neural Progenitors, and Engineered Stem Cells -- Herpes Simplex Virus Type 1 (HSV-1)-Derived Recombinant Vectors for Gene Transfer and Gene Therapy -- Herpes Simplex Virus Type 1 (HSV-1)-Derived Amplicon Vectors for Gene Transfer and Gene Therapy -- Bone Marrow Transplantation for Research and Regenerative Therapies in the Central Nervous System -- Detection of Activated Caspase-8 in Injured Spinal Axons by Using Fluorochrome-Labeled Inhibitors of Caspases (FLICA) -- Generation of Zebrafish Models by CRISPR/Cas9 Genome Editing -- In Vivo Assessment of Neuronal Cell Death in Drosophila -- Drosophila Model for Studying Phagocytosis Following Neuronal Cell Death. | |
| 520 | _aThis volume represents a valuable and readily reproducible collection of established and emerging techniques for neuronal cell death research. Conveniently divided into four parts, sections cover a series of techniques for the molecular, structural, functional and genomic characterization of dying neurons, a number of protocols that are of primary interest in neuropathology and in experimental neuropathology, a series of gene engineering techniques to obtain and manipulate neuronal stem cells and progenitors, to prepare HSV-1 vectors for the gene therapy, and to CNS transplantation of bone marrow stem cells, and finally, some very interesting protocols for the study of cell death in non-mammalian models. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Neuronal Cell Death: Methods and Protocols seeks to serve a large audience of scientists that are currently active in the field or are willing to enter such an exciting and still expanding area of neurobiology. | ||
| 700 | 1 |
_aLossi, Laura _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt |
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| 700 | 1 |
_aMerighi, Adalberto _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt |
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| 776 | 0 | 8 |
_iPrinted edition: _z9781493921539 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781493921515 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781493944644 |
| 856 | 4 | 0 |
_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2152-2 _z(usuarios Universidad Europea de Valencia) |
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| 988 | _aSpringer_Protocols_2015 | ||
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