000			04650nam a22003855i 4500
001			235358
003			ES-VaUE
005			20221220020700.0
007			cr nn 008mamaa
008			160811s2016 xxu| s |||| 0|eng d
020			_a9781493963522
024	7		_a10.1007/978-1-4939-6352-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHigh-Resolution Imaging of Cellular Proteins _bMethods and Protocols _cedited by Steven D. Schwartzbach, Omar Skalli, Thomas Schikorski.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XIII, 366 páginas) _b45 ilustraciones, 21 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1474
505	0		_aExpression of Epitope-Tagged Proteins in Mammalian Cells in Culture -- Antibody Production with Synthetic Peptides -- Production and Purification of Polyclonal Antibodies -- Preparation of Colloidal Gold Particles and Conjugation to Protein A/G/L, IgG, F(ab')2 and Streptavidin -- Helper Dependent Adenoviral Vectors and their use for Neuroscience Applications -- Localizing Proteins in Fixed Giardia Lamblia and Live Cultured Mammalian Cells by Confocal Fluorescence Microscopy -- Using Fluorescent Protein Fusions to Study Protein Subcellular Localization and Dynamics in Plant Cells -- Using FRAP Or FRAPA to Visualize the Movement of Fluorescently-Labeled Proteins or Cellular Organelles in Live Cultured Neurons Transformed with Adeno-Associated Viruses -- Bimolecular Fluorescence Complementation (BiFc) Analysis of Protein-Protein Interactions and Assessment of Subcellular Localization in Live Cells -- Viral Injection and Cranial Window Implantation for In Vivo Two-Photon Imaging -- Imaging Synaptic Vesicle Exocytosis-Endocytosis with pH Sensitive Fluorescent Proteins -- Immunogold Protein Localization on Grid-Glued Freeze-Fracture Replicas -- Pre-Embedding Double-Label Immunoelectron Microscopy of Chemically Fixed Tissue Culture Cells -- Immunoelectron Microscopy of Cryofixed and Freeze- Substituted Plant Tissues -- Immunoelectron Microscopy of Cryofixed Freeze Substituted Yeast Saccharomyces cerevisiae -- Pre-embedding Method of Electron Microscopy for Glycan Localization in Mammalian Tissues and Cells Using Lectin Probes -- Pre-Embedding Nanogold Silver and Gold Intensification -- Post-Embedding Mammalian Tissue for Immunoelectron Microscopy: A Standardized Procedure Based On Heat-Induced Antigen Retrieval -- Pre- and Post-Embedding Immunogold Labeling of Tissue Sections -- Immuno-Gold Labelling for Scanning Electron Microscopy -- Monitoring Synaptic Vesicle Protein Sorting with Enhanced Horseradish Peroxidase in the Electron Microscope -- Functional Nanoscale Imaging Of Synaptic Vesicle Cycling With Superfast Fixation.
520			_aThis volume presents authoritative and cutting-edge methods and protocols focusing on three tool boxes covering the increasingly diverse methodologies used to image selected proteins and to investigate their function by light and electron microscopy. The first tool box includes the development of a wide range of molecular and immunological probes to target specific proteins. The second details the use of these probes for high resolution fluorescence microscopy and the third focuses on applications for transmission and scanning electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls thus ensuring successful results in the further study of this vital field.
700	1		_aSchwartzbach, Steven D _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aSkalli, Omar _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aSchikorski, Thomas _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493963508
776	0	8	_iPrinted edition: _z9781493963515
776	0	8	_iPrinted edition: _z9781493981731
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6352-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c235358 _d235358