000			04600nam a22003615i 4500
001			235372
003			ES-VaUE
005			20221220020701.0
007			cr nn 008mamaa
008			140502s2014 xxu| s |||| 0|eng d
020			_a9781493907007
024	7		_a10.1007/978-1-4939-0700-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPlant Circadian Networks _bMethods and Protocols _cedited by Dorothee Staiger.
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XI, 361 páginas) _b77 ilustraciones, 47 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1158
505	0		_aMeasurement of Luciferase Rhythms -- Online Period Estimation and Determination of Rhythmicity in Circadian Data, using the BioDare Data Infrastructure -- Global profiling of the circadian transcriptome using microarrays -- ChIP-Seq analysis of histone modifications at the core of the Arabidopsis circadian clock -- Quantitative transcriptome analysis using RNA-seq -- Rapid and parallel quantification of small and large RNA species -- The RIP per case - identification of RNA binding protein targets by RNA immunoprecipitation -- A protocol for visual analysis of alternative splicing in RNA-Seq data using Integrated Genome Browser -- AthaMap web tools for the analysis of transcriptional and post-transcriptional gene expression regulation in Arabidopsis thaliana -- Analysis of mRNA translation states in Arabidopsis over the diurnal cycle by polysome microarray -- Immunoprecipitation-based analysis of protein-protein interactions -- Comparative Phosphoproteomics to Identify Targets of the Clock-Relevant Casein Kinase 1 in C. reinhardtii Flagella -- Pulsed induction of circadian clock genes in Arabidopsis seedlings -- The use of fluorescent proteins to analyze circadian rhythms -- Measuring circadian oscillations of cytosolic-free calcium in Arabidopsis thaliana -- Circadian life without micronutrients: effects of altered micronutrient supply on clock function in Arabidopsis -- Assessing redox state and reactive oxygen species in circadian rhythmicity -- Circadian regulation of plant immunity to pathogens -- Determination of photoperiodic flowering time control in Arabidopsis and barley -- The perennial clock is an essential timer for seasonal growth events and cold hardiness -- Monitoring seasonal bud set, bud burst and cold hardiness in Populus -- Transformation and Measurement of Bioluminescence Rhythms in the Moss Physcomitrella patens -- Modeling and Simulating the Arabidopsis thaliana Circadian Clock Using XPP-AUTO.
520			_aPlant Circadian Networks: Methods and Protocols provides a collection of protocols to investigate clock-controlled parameters including transcript and small RNA levels, promoter activity using luciferase reporters, protein levels and posttranslational modification, protein-protein interaction, in vivo DNA-protein interaction and RNA-protein interaction, cellular redox state, Ca2+ levels, and innate immune responses. Furthermore, the use of bioinformatics resources is described to evaluate high throughput data sets and to integrate the data into an overarching picture of circadian networks in the cell. Additional chapters focus on seasonal processes like flowering time control, and techniques on trees, moss and algae.   Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Plant Circadian Networks: Methods and Protocols is designed not only for the chronobiology community dealing with circadian biology but also for the plant community in general.
700	1		_aStaiger, Dorothee _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493907014
776	0	8	_iPrinted edition: _z9781493906994
776	0	8	_iPrinted edition: _z9781493945870
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-0700-7 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c235372 _d235372