000			03605nam a22003615i 4500
001			235377
003			ES-VaUE
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007			cr nn 008mamaa
008			110322s2011 xxu| s |||| 0|eng d
020			_a9781617790836
024	7		_a10.1007/978-1-61779-083-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMicroRNAs in Development _bMethods and Protocols _cedited by Tamas Dalmay.
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (X, 200 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v732
505	0		_aIn situ Detection of microRNAs in Animals -- Detection of microRNAs in Plants by In situ Hybridization -- Detecting Animal and Plant microRNAs by Northern Blotting -- Profiling microRNAs by Real-time PCR -- Detection of Small RNAs and microRNAs using Deep Sequencing Technology -- Detection of microRNAs in Prostate Cancer Cells by microRNA Array -- microRNA Knock Down by Cholesterol Conjugated Antisense Oligos in Mouse Organ Culture -- Protocols for Use of Homologous Recombination Gene-targeting to Produce microRNA Mutants in Drosophila -- Engineering Elements for Gene Silencing: The Artificial microRNAs Technology -- Mimicry Technology: Suppressing Small RNA Activity in Plants -- Experimental Validation of MicroRNA Targets using a Luciferase Reporter System -- Experimental Identification of microRNA Targets by Immunoprecipitation of Argonaute Protein Complexes -- Comprehensive Identification of miRNA Target Sites in Live Animals -- Target Validation of Plant microRNAs.- A High-throughput Sequencing-based Methodology to Identify All Uncapped and Cleaved RNA Molecules in Eukaryotic Genomes.
520			_aSince the discovery of microRNAs, developmental biologists have striven to understand the role of miRNAs in development and disease. MicroRNAs in Development: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Divided into three convenient sections, this detailed volume covers various techniques to detect and profile miRNA expression, followed by protocols to manipulate the activity of miRNAs in various organisms, and it concludes with a section that outlines different methods to identify and validate miRNA targets in animals and plants. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and accessible, MicroRNAs in Development: Methods and Protocols serves as a practical guide for scientists of all backgrounds and conveys the appropriate sense of fascination associated with this vital field of research.
700	1		_aDalmay, Tamas _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617790829
776	0	8	_iPrinted edition: _z9781617790843
776	0	8	_iPrinted edition: _z9781493957941
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-083-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2011
999			_c235377 _d235377