000			04919nam a22003735i 4500
001			235385
003			ES-VaUE
005			20221220020701.0
007			cr nn 008mamaa
008			100301s1998 xxu| s |||| 0|eng d
020			_a9781592595976
024	7		_a10.1385/0896034852 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMolecular Diagnosis of Infectious Diseases _cedited by Udo Reischl.
250			_a1st edition 1998
264		1	_aTotowa, NJ _bHumana Press _c1998
300			_a1 recurso en línea (XV, 629 páginas) _b126 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v13
505	0		_aRecombinant Proteins and Peptide -- Identification of Novel Diagnostic Markers by Differential Display -- Immunoprecipitation -- RT-PCR Methods and Applications -- Quantitative RT-PCR -- Cloning cDNA Ends Using RACE -- A Method for the Rapid Identification of Epitopes and Other Functional Peptide Domains -- Mapping of Linear B-Cell Epitopes on Viral Polypeptides by Multiple Peptide Synthesis and Fine Tuning Sensitivity and Specificity of the Identified Peptide Antigens for Application in Virus Diagnosis -- Amplification of Genomic DNA by PCR -- PCR-Based Cloning and Subsequent Expression of Antigenic Proteins in Escherichia coli -- Application of TEV Protease in Protein Production -- Stable Transfected Drosophila Schneider-2 Cells as a Novel Tool to Produce Recombinant Antigens for Diagnostic, Therapeutic, and Preventive Purposes -- Use of Recombinant Antigens as Diagnostic Reagents in Enzyme-Linked Immunosorbent Assays -- Generation of Recombinant Baculovirus DNA in E.coli Using a Baculovirus Shuttle Vector -- The SFV Gene Expression System -- Expression and Purification of Recombinant Proteins Using the pET System -- Procedures for the Analysis and Purification of His-Tagged Proteins -- Detection and Immobilization of Proteins Containing the 6xHis Tag -- The Use of Recombinant Fusion Proteases in the Affinity Purification of Recombinant Proteins -- Purification and Immunological Characterization of Recombinant Antigens Expressed in the Form of Insoluble Aggregates (Inclusion Bodies) -- Purification of Recombinant Proteins Having High Isoelectric Points -- Immunoblotting -- Diagnostic Application of a Multiple-Channel Immunoblot Apparatus -- Immobilization of lmmunoreactants on Solid Phases for ELlSAs -- Nonradioactive Labeling and lmmunodetection of Recombinant Proteins -- Flow Cytometry -- Basic Problems of Serological Laboratory Diagnosis -- Molecular Diagnostics Resources on the Internet -- Antibodies -- Monoclonal Antibody-Based Immunoassays -- Biosensor-Based Methods in Clinical Diagnosis -- The Quartz Crystal Microbalance (QCM) as an Immunosensor -- Biomolecular Interaction Analysis -- Recent Developments in Antibody Engineering -- Cloning and Expression of Single-Chain Fragments (scFv) from Mouse and Rat Hybridomas -- Production of a Human Antibody Library in the Phage-Display Vector pSEX81 -- Screening of Phage-Displayed Antibody Libraries -- Purification, Characterization, and Biotinylation of Single-Chain Antibodies.
520			_aIn Molecular Diagnosis of Infectious Diseases leading international authorities provide a comprehensive and up-to-date set of basic and advanced methods that illuminate the principles of molecular diagnostics and allow researchers to establish assay systems tailored to their specific needs. This unique collection of protocols covers the latest strategies for identifying novel diagnostic markers, epitope mapping, and the production and purification of recombinant antigens, as well as their use as diagnostic reagents in immunological assays. It also includes promising techniques based on recent developments in antibody engineering and on the use of biosensors. Molecular Diagnosis of Infectious Diseases offers clinical microbiologists, clinical chemists, and pathologists unique tools with which to develop improved assays for diagnosing infectious diseases. The book is certain to play a leading role in helping researchers to understand and realize in their daily work the powerful potential of molecular diagnostic techniques in clinical microbiology today.
700	1		_aReischl, Udo _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489943019
776	0	8	_iPrinted edition: _z9780896034853
776	0	8	_iPrinted edition: _z9781489943002
776	0	8	_iPrinted edition: _z9780896033986
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896034852 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1998
999			_c235385 _d235385