000			04948nam a22003495i 4500
001			235388
003			ES-VaUE
005			20221220020702.0
007			cr nn 008mamaa
008			100301s1996 xxu| s |||| 0|eng d
020			_a9781592595402
024	7		_a10.1385/0896033198 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aYeast Protocols _bMethods in Cell and Molecular Biology _cedited by Ivor H. Evans.
250			_a1st edition 1996
264		1	_aTotowa, NJ _bHumana Press _c1996
300			_a1 recurso en línea (XIII, 433 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v53
505	0		_aIsolation and Identification of Yeasts from Natural Habitats -- Maintenance and Culture of Yeasts -- Mutagenesis in Yeast -- Rare-Mating and Cytoduction in Saccharomyces cerevisiae -- Protoplast Fusion in Saccharomyces cerevisiae -- Meiotic Analysis -- Ascus Dissection -- Karyotyping of Yeast Strains by Pulsed-Field Gel Electrophoresis -- Chromosomal Localization of Genes Through Pulsed-Field Gel Electrophoresis Techniques -- Isolation, Purification, and Analysis of Nuclear DNA in Yeast Taxonomy -- Isolation of Yeast DNA -- Isolation of Mitochondrial DNA -- Isolation of Yeast Plasma Membranes -- The Extraction and Analysis of Sterols from Yeast -- Peroxisome Isolation -- Transformation of Lithium-Treated Yeast Cells and the Selection of Auxotrophic and Dominant Markers -- Biolistic Transformation of Yeasts -- Genomic Yeast DNA Clone Banks -- Yeast Colony Hybridization -- Determination of Plasmid Copy Number in Yeast -- Determination of Chromosome Ploidy in Yeast -- Chromosome Engineering in Yeast with a Site-Specific Recombination System from a Heterologous Yeast Plasmid -- Insertional Mutagenesis by Ty Elements in Saccharomyces cerevisiae -- Reporter Gene Systems for Assaying Gene Expression in Yeast -- Preparation and Use of Yeast Cell-Free Translation Lysate -- Electrophoretic Analysis of Yeast Proteins -- Preparation of Total RNA -- mRNA Abundance and Half-Life Measurements -- Polysome Analysis -- Induction of Heat Shock Proteins and Thermotolerance -- Rhodamine B Assay for Estimating Activity of Killer Toxins Permeabilizing Cytoplasmic Membranes -- Nystatin-Rhodamine B Assay for Estimating Activity of Killer Toxin from Kluyveromyces lactis -- Application of Killer Toxins in Stepwise Selection of Hybrids and Cybrids Obtained by Induced Protoplast Fusion -- Killer Plaque Technique for Selecting Hybrids and Cybrids Obtained by Induced Protoplast Fusion -- Genotoxicity Testing in Schizosaccharomyces pombe -- Purification and Quantification of Saccharomyces cerevisiae Cytochrome P450 -- Calorimetry of Whole Yeast Cells -- Light Microscopy Methods -- Fluorescence Microscopy Methods -- Immunoelectron Microscopy.
520			_aYeast Protocols intends to offer a selection of well-proven proto­ cols in cell and molecular biology, applicable to yeasts including, but certainly not exclusively aimed at, Saccharomyces cerevisiae. Saccharomyces cerevisiae and its very distant cousin, Schizosacc- romyces pombe, are of course now foremost model eukaryotic organisms, and the focus of wide-ranging experimental studies, especially those using molecular genetic techniques. Many of the lat­ ter, such as DNA sequencing and in vitro mutagenesis, are general DNA techniques, well covered in other volumes of Humana's Meth­ ods in Molecular Biology series, and elsewhere. The inclusion of a number of non-DNA techniques in this book is meant to reflect the resurgent interest in yeast cell biology sparked by the development of gene manipulation methods-for example, cellular localization of cloned gene products using microscopical techniques. The presentation of protocols follows the successful Methods in Molecular Biology series format, with a clear sequence of steps and extensive troubleshooting notes. It is our hope that these protocols will be useful not only to established members of the full-time research community, but also to the less experienced-first degree level and masters students undertaking project work, as well as PhD students starting their experimental programs; I am well aware that these young apprentice scientists are not always able to receive the supervision time that they, and indeed their supervisors, would like.
700	1		_aEvans, Ivor H _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9780896033191
776	0	8	_iPrinted edition: _z9781489940414
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896033198 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1996
999			_c235388 _d235388