000			03359nam a22003615i 4500
001			235391
003			ES-VaUE
005			20221220020702.0
007			cr nn 008mamaa
008			100301s2002 xxu| s |||| 0|eng d
020			_a9781592592791
024	7		_a10.1385/1592592791 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aApoptosis Methods in Pharmacology and Toxicology _bApproaches to Measurement and Quantification _cedited by Myrtle A. Davis.
250			_a1st edition 2002
264		1	_aTotowa, NJ _bHumana Press _c2002
300			_a1 recurso en línea (XIV, 156 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Pharmacology and Toxicology _x1940-6053
505	0		_aFlow Cytometric and Fluorometric Methods of Quantifying and Characterizing Apoptotic Cell Death -- Analysis of Apoptosis by Laser-Scanning Cytometry -- Specific Methods for Detection and Quantification of Apoptosis in Tissue Sections -- DNA Microarrays -- Microarray Analysis of Apoptosis -- ELISAs for Quantification of Bcl-2 Family Activities and Active Caspases.
520			_aApoptosis, or programmed cell death, is a widespread cellular process that regulates numerous important biological events ranging from the metamorphosis of the tadpole tail to the elimination of surplus brain cells in the formation of proper synaptic connections. In Apoptosis Methods in Pharmacology and Toxicology: Approaches to Measurement and Quantification, Dr. Myrtle A. Davis has assembled a panel of cutting-edge scientists to describe their best methods for detecting, illuminating, and quantifying apoptotic mechanisms in a way that is useful in toxicology and pharmacology research. These state-of-the-art techniques include flow cytometric, fluorometric, and laser scanning methods for quantifying and characterizing apoptosis, as well as protocols for the use of DNA microarray technology, high-throughput screens, and ELISA. Immunocytochemical methods for measuring biochemical and molecular endpoints in tissue sections will be highly useful for those carrying out studies in whole animal models as opposed to cell culture systems. Presented by authors well-versed in the technical problems and challenges in analyzing apoptosis, each method explicates its successful use, describes its limitations, and shows how it may be applied in large-scale screening operations. Concise and eminently practical, Apoptosis Methods in Pharmacology and Toxicology: Approaches to Measurement and Quantification offers pharmacologists, toxicologists, pathologists, and many other biomedical scientists today's gold standard reference source for methods that definitively identify and accurately quantify apoptosis.
700	1		_aDavis, Myrtle A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617372391
776	0	8	_iPrinted edition: _z9780896038905
776	0	8	_iPrinted edition: _z9781489943682
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592791 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2002
999			_c235391 _d235391