000			03976nam a22003735i 4500
001			235401
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007			cr nn 008mamaa
008			161011s2017 xxu| s |||| 0|eng d
020			_a9781493964123
024	7		_a10.1007/978-1-4939-6412-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aProtein Chromatography _bMethods and Protocols _cedited by Dermot Walls, Sinéad T. Loughran.
250			_a2nd edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (X, 426 páginas) _b40 ilustraciones, 21 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1485
505	0		_aA Synopsis of Protein and their Purification -- Gel-Filtration Chromatography -- Immunoaffinity Chromatography: Concepts and Applications -- Avoiding Proteolysis During Protein Purification -- Scale-Up of Protein Purification: Downstream Processing Issues -- Phage Display: A Powerful Technology for the Generation of High Specificity Affinity Reagents from Alternative Immune Sources -- Protein Stability: Enhancement and Measurement -- Tagging Recombinant Proteins to Enhance Solubility and Aid Purification -- Storage and Lyophilisation of Pure Proteins -- Differential Precipitation and Solubilization of Proteins -- Ion-Exchange Chromatography: Basic Principles and Application -- Protein Quantitation and Analysis of Purity -- Purification of Proteins Fused to Maltose-Binding Protein -- Purification of Poly-Histidine-Tagged Proteins -- Purification of Antibodies Using Affinity Chromatography -- Optimized Generation of High-Affinity, High-Specificity Single-Chain Fv Antibodies from Multi-Antigen Immunized Chickens -- Measuring Protein-Protein Interactions Using Biacore -- Hydrophobic Interaction Chromatography. .
520			_aThis second edition expands on the previous edition with new chapters that are suitable for newcomers, as well as more detailed chapters that cover protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the challenges that scale-up of production poses to the investigator. Many of the chapters also discuss generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins as a means to enhance their solubility and simplify their purification on an individual scale or in high-throughput systems. This book also provides readers with chapters that describe not just the more commonly used methods, but also recently developed approaches such as proteomic/mass spectrometric techniques and Lectin-based affinity chromatography. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein Chromatography: Methods and Protocols, Second Edition is a valuable resource for anyone who is interested in the field of protein chromatography.
700	1		_aWalls, Dermot _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aLoughran, Sinéad T _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493964109
776	0	8	_iPrinted edition: _z9781493964116
776	0	8	_iPrinted edition: _z9781493981915
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6412-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c235401 _d235401