000			03457nam a22003615i 4500
001			235402
003			ES-VaUE
005			20221220020703.0
007			cr nn 008mamaa
008			100301s1999 xxu| s |||| 0|eng d
020			_a9781592596836
024	7		_a10.1385/1592596835 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aNMDA Receptor Protocols _cedited by Min Li.
250			_a1st edition 1999
264		1	_aTotowa, NJ _bHumana Press _c1999
300			_a1 recurso en línea (X, 196 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v128
505	0		_aIsolation of Receptor Clones by Expression Screening in Xenopus Oocytes -- Expression of NMDA Receptor Channel Subunit Proteins Using Baculovirus and Herpesvirus Vectors -- Transient Expression of Functional NMDA Receptors in Mammalian Cells -- Stable Expression of Human NMDA Receptors in Cultured Mammalian Cells -- Expression of Extracellular N-Terminal Domain of NMDA Receptor in Mammalian Cells -- Immunocytochemistry of NMDA Receptors -- The Use of Ligand Binding in Assays of NMDA Receptor Function -- Calmodulin Modification of NMDA Receptors -- Detergent Solubilization and Immunoprecipitation of Native NMDA Receptors -- Redox Sensitivity of NMDA Receptors -- In Vitro Selection of Peptides Acting on NMDA Receptors -- NMDA Receptor Pharmacology and Analysis of Patch-Clamp Recordings -- Determination of Membrane Topology of Glutamate Receptors -- Engineering the NMDA Receptor Channel Lining -- High-Throughput Functional Detection of NMDA Receptor Activity.
520			_aIn NMDA Receptor Protocols, Min Li and a panel of hands-on experimentalists detail state-of-the-art molecular techniques for studying NMDA ligand-gated ion channels and developing assays for nontherapeutic lead selection. The topics range from cDNA cloning to in vitro and in vivo investigation of the channel complex in the mammalian brain. Additional topics include the biochemical analysis of the channel protein and the construction of various heterologous systems for both basic research and high throughput screens (HTS) for pharmaceutical chemicals. Although the focus is on NMDA receptors, the methods are applicable to other ligand-gated ion channels and with some modification may be extended to related membrane receptors or signaling receptors. NMDA Receptor Protocols provides today's scientists at all skill levels a diverse collection of highly reproducible molecular techniques for ion channel research and biotherapeutic development. Eminently practical and reproducible, these techniques offer powerful methods for basic research on NMDA receptor structure and function, as well as enormous opportunities for clinical investigation toward the development of novel bioactive compounds.
700	1		_aLi, Min _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617371462
776	0	8	_iPrinted edition: _z9780896037137
776	0	8	_iPrinted edition: _z9781489943613
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592596835 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1999
999			_c235402 _d235402