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001			235409
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007			cr nn 008mamaa
008			100301s2004 xxu| s |||| 0|eng d
020			_a9781592598229
024	7		_a10.1385/1592598226 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCell Cycle Control and Dysregulation Protocols _cedited by Antonio Giordano, Gaetano Romano.
250			_a1st edition 2004
264		1	_aTotowa, NJ _bHumana Press _c2004
300			_a1 recurso en línea (XIII, 185 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v285
505	0		_aAnalysis of Cyclins and Cyclin-Dependent Kinases -- The Biology of Cyclins and Cyclin-Dependent Protein Kinases -- In Situ Immunofluorescence Analysis -- In Situ Immunofluorescence Analysis -- Immunofluorescence Analysis Using Epitope-Tagged Proteins -- Analysis of In Vivo Gene Expression Using Epitope-Tagged Proteins -- Chromatin Immunoprecipitation -- Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expression -- Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expression -- Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expression -- Assays for Cyclin-Dependent Kinase Inhibitors -- Protein Degradation Via the Proteosome -- Analysis of the Factors Involved in Cell Cycle Deregulation -- The Transformed Phenotype -- A Morphologic Approach to Detect Apoptosis Based on Electron Microscopy -- Detection of Apoptotic Deoxyribonucleic Acid Break by In Situ Nick Translation -- Cellular Response to Deoxyribonucleic Acid Damage -- Induction of Deoxyribonucleic Acid Damage by Alkylating Agents -- Induction of Deoxyribonucleic Acid Damage by ? Irradiation -- Ultraviolet Irradiation of Cells -- Retroviridae-Based Vectors: Protocols for Lentiviral- and Retroviral-Mediated Gene Transfer to Engineer Cell Culture Systems -- Transient Production of Retroviral- and Lentiviral-Based Vectors for the Transduction of Mammalian Cells -- Determination of Functional Viral Titer by Drug-Resistance Colony Assay, Expression of Green Fluorescent Protein, and ?-Galactoside Staining -- Retroviral and Lentiviral Vector Titration by the Analysis of the Activity of Viral Reverse Transcriptase -- Detection of Gene Expression in Subcellular Compartments -- Single and Double Colloidal Gold Labeling in Postembedding Immunoelectron Microscopy -- Multifluorescence Labeling and Colocalization Analyses.
520			_aThe dysregulation of the normal cell cycle underlies an enormous number of pathological conditions, including the neurodegenerative disorders, all cancers, and diabetes. In Cell Cycle Control and Dysregulation Protocols: Cyclins, Cyclin-Dependent Kinases, and Other Factors, expert laboratorians detail emerging methodologies for studying the cell cycle, its kinases, and kinase inhibitors. The authors focus on issues of gene expression in vivo and in vitro, the analysis of cyclin-dependent kinase inhibitors, protein degradation mediated by the proteosome, the analysis of the transformed cell phenotype, and innovative techniques to detect apoptosis. Many of the protocols are new, such as electron microsocopy to detect apoptosis and proteosome-mediated degradation, whereas others study the interactions between protein-protein, protein-DNA, and protein-RNA. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Cell Cycle Control and Dysregulation Protocols: Cyclins, Cyclin-Dependent Kinases, and Other Factors offers pathbreaking researchers powerful tools for probing the cell cycle regulation across a wide variety of pathologies.
700	1		_aGiordano, Antonio _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aRomano, Gaetano _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617372704
776	0	8	_iPrinted edition: _z9780896039490
776	0	8	_iPrinted edition: _z9781489939456
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592598226 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2004
999			_c235409 _d235409