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001			235414
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007			cr nn 008mamaa
008			100301s2009 xxu| s |||| 0|eng d
020			_a9781603279772
024	7		_a10.1007/978-1-60327-977-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aZebrafish _bMethods and Protocols _cedited by Graham J. Lieschke, Andrew C. Oates, Koichi Kawakami.
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XI, 335 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v546
505	0		_aMutagenesis and Mutants -- Highly Efficient ENU Mutagenesis in Zebrafish -- Production of Pseudotyped Retrovirus and the Generation of Proviral Transgenic Zebrafish -- Undertaking a Successful Gynogenetic Haploid Screen in Zebrafish -- Cryopreservation and In Vitro Fertilization at the Zebrafish International Resource Center -- Transgenesis -- Transient and Stable Transgenesis Using Tol2 Transposon Vectors -- Analysis of Genes and Genome by the Tol2-Mediated Gene and Enhancer Trap Methods -- Bacterial Artificial Chromosome Transgenesis for Zebrafish -- Simple and Efficient Transgenesis with Meganuclease Constructs in Zebrafish -- Tissue-Specific Manipulations -- Nitroreductase-Mediated Cell Ablation in Transgenic Zebrafish Embryos -- Focal Electroporation in Zebrafish Embryos and Larvae -- Tissue Micromanipulation in Zebrafish Embryos -- Analyzing Gene Expression -- Zebrafish Spotted-Microarray for Genome-Wide Expression Profiling Experiments. Part I: Array Printing and Hybridization -- Zebrafish Spotted-Microarray for Genome-Wide Expression Profiling Experiments: Data Acquisition and Analysis -- Validating microRNA Target Transcripts Using Zebrafish Assays -- Imaging -- Multiple Embryo Time-Lapse Imaging of Zebrafish Development -- Live Cell Imaging of Zebrafish Leukocytes -- Imaging Zebrafish Embryos by Two-Photon Excitation Time-Lapse Microscopy -- Morphological Analysis of the Zebrafish Digestive System -- In Toto Imaging of Embryogenesis with Confocal Time-Lapse Microscopy.
520			_aIn the last 20 years, research activity using the zebrafish Danio rerio has increased dramatically, due in part to the ease of breeding and raising them, their genetic tractability, embryonic accessibility, and their imaging potential. In Zebrafish: Methods and Protocols, expert researchers provide a collection of experimental techniques for the genetic modification, manipulation, and in vivo live imaging of zebrafish. This volume collects sophisticated and practical techniques, including several not yet widely known, from laboratories around the world. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Zebrafish: Methods and Protocols seeks to aid and inspire scientists utilizing this versatile animal model in their research.
700	1		_aLieschke, Graham J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aOates, Andrew C _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aKawakami, Koichi _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781607613381
776	0	8	_iPrinted edition: _z9781603279765
776	0	8	_iPrinted edition: _z9781627038997
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-977-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2009
999			_c235414 _d235414