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008			100301s2007 xxu| s |||| 0|eng d
020			_a9781597455138
024	7		_a10.1007/978-1-59745-513-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
100	1		_aMcIntosh, Thomas J. _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut
245	1	0	_aLipid Rafts _cby Thomas J. McIntosh.
250			_a1st edition 2007
264		1	_aTotowa, NJ _bHumana Press _c2007
300			_a1 recurso en línea (XI, 326 páginas) _b112 ilustraciones, 8 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v398
505	0		_aOverview of Membrane Rafts -- Analysis of Raft Affinity of Membrane Proteins by Detergent-Insolubility -- Nondetergent Isolation of Rafts -- Detecting Ordered Domain Formation (Lipid Rafts) in Model Membranes Using Tempo -- Using Monomolecular Films to Characterize Lipid Lateral Interactions -- Electro-Formation and Fluorescence Microscopy of Giant Vesicles With Coexisting Liquid Phases -- Fluorescence Correlation Spectroscopy -- Multiphoton Laser-Scanning Microscopy and Spatial Analysis of Dehydroergosterol Distributions on Plasma Membrane of Living Cells -- NMR Detection of Lipid Domains -- Lateral Diffusion Coefficients of Raft Lipids From Pulsed Field Gradient NMR -- Saturation-Recovery Electron Paramagnetic Resonance Discrimination by Oxygen Transport (DOT) Method for Characterizing Membrane Domains -- Plasmon-Waveguide Resonance Spectroscopy Studies of Lateral Segregation in Solid-Supported Proteolipid Bilayers -- Fluorescence Recovery After Photobleaching Studies of Lipid Rafts -- Single-Molecule Tracking -- X-Ray Diffraction to Determine the Thickness of Raft and Nonraft Bilayers -- Small-Angle Neutron Scattering to Detect Rafts and Lipid Domains -- Exploring Membrane Domains Using Native Membrane Sheets and Transmission Electron Microscopy -- Atomic Force Microscopy of Lipid Domains in Supported Model Membranes -- Atomistic and Coarse-Grained Computer Simulations of Raft-Like Lipid Mixtures -- A Microscopic Model Calculation of the Phase Diagram of Ternary Mixtures of Cholesterol and Saturated and Unsaturated Phospholipids.
520			_aIn the past several years significant attention has been given to the analysis of the properties and functions of lateral microdomains (rafts) in biological membranes. As described in the overview chapter of this book, as well as in the introductions to many of the other chapters, there are many fundamental un- swered questions concerning the composition, structure, dynamics, and even the very existence of these membrane rafts. Therefore, a variety of sophisticated techniques have been used to study intact cell membranes, as well as model s- tems composed of specific lipids and proteins thought to be in rafts. The analyses of biological membranes have provided detailed data on intact microdomains, and the complementary studies of model systems have given critical insights on the roles of specific lipid-lipid and lipid-protein interactions in raft formation and function. The chapters in this book provide detailed information on modern methods that are currently being employed to study lipid rafts.
776	0	8	_iPrinted edition: _z9781617377440
776	0	8	_iPrinted edition: _z9781588297297
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-513-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2007
999			_c235428 _d235428