000			03618nam a22003615i 4500
001			235431
003			ES-VaUE
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007			cr nn 008mamaa
008			100301s1999 xxu| s |||| 0|eng d
020			_a9781592597222
024	7		_a10.1385/159259722X _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aConfocal Microscopy _bMethods and Protocols _cedited by Stephen W. Paddock.
250			_a1st edition 1999
264		1	_aTotowa, NJ _bHumana Press _c1999
300			_a1 recurso en línea (XII, 446 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v122
505	0		_aAn Introduction to Confocal Imaging -- Practical Considerations for Collecting Confocal Images -- Fluorescent Probes for Confocal Microscopy -- Imaging Gene Expression Using Antibody Probes -- Single and Double FISH Protocols for Drosophila -- Confocal Microscopy of Plant Cells -- Preparation of Yeast Cells for Confocal Microscopy -- Confocal Methods for Caenorhabditis elegans -- Imaging Sea Urchin Fertilization -- Imaging Immunolabeled Drosophila Embryos by Confocal Microscopy -- Confocal Microscopy on Xenopus laevis Oocytes and Embryos -- Analyzing Morphogenetic Cell Behaviors in Vitally Stained Zebrafish Embryos -- Confocal Imaging of Living Cells in Intact Embryos -- Live Confocal Analysis with Fluorescently Labeled Proteins -- Live Imaging with Green Fluorescent Protein -- Fluorescent Calcium Indicators:Subcellular Behavior and Use in Confocal Imaging -- Intracellular pH and pCa Measurement -- Measuring Dynamic Cell Volume In Situ by Confocal Microscopy -- Imaging Thick Tissues with Confocal Microscopy -- Measurement in the Confocal Microscope -- Presentation of Confocal Images -- The Preparation of Stereoscopic 3D Illustrations of Confocal Data Sets for Publications and Slides -- Information Management of Confocal Microscopy Images -- Morphing Confocal Images and Digital Movie Production.
520			_aIn Confocal Microscopy Methods and Protocols, Stephen Paddock and a highly skilled panel of experts lead the researcher using confocal techniques from the bench top, through the imaging process, to the journal page. They concisely describe all the key stages of confocal imaging-from tissue sampling methods, through the staining process, to the manipulation, presentation, and publication of the realized image. Written in a user-friendly, nontechnical style, the methods specifically cover most of the commonly used model organisms: worms, sea urchins, flies, plants, yeast, frogs, and zebrafish. Centered in the many biological applications of the confocal microscope, the book makes possible the successful imaging of both fixed and living specimens using primarily the laser scanning confocal microscope. The powerful hands-on methods collected in Confocal Microscopy Methods and Protocols will help even the novice to produce first-class cover-quality confocal images.
700	1		_aPaddock, Stephen W _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489943392
776	0	8	_iPrinted edition: _z9780896035263
776	0	8	_iPrinted edition: _z9781617370625
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/159259722X _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1999
999			_c235431 _d235431