000			03628nam a22003735i 4500
001			235439
003			ES-VaUE
005			20221220020705.0
007			cr nn 008mamaa
008			100301s2008 xxu| s |||| 0|eng d
020			_a9781603271691
024	7		_a10.1007/978-1-60327-169-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMesenchymal Stem Cells _bMethods and Protocols _cedited by Darwin J. Prockop, Donald G. Phinney, Bruce A. Bunnell.
250			_a1st edition 2008
264		1	_aTotowa, NJ _bHumana Press _c2008
300			_a1 recurso en línea (XVI, 192 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v449
505	0		_aIsolation of Human Multipotential Stromal Cells from Bone Marrow and Adipose Tissue -- Isolation and Culture of Bone Marrow-Derived Human Multipotent Stromal Cells (hMSCs) -- Mesenchymal Stem Cells from Adult Bone Marrow -- A Method to Isolate and Purify Human Bone Marrow Stromal Stem Cells -- Adipose-Derived Stem Cells -- Isolation of Human Adipose-derived Stem Cells from Biopsies and Liposuction Specimens -- Manipulation of Human Multipotential Stromal Cells -- Colony Forming Unit Assays for MSCs -- Differentiation and Characterization of Human MSCs -- Freezing Harvested hMSCs and Recovery of hMSCs from Frozen Vials for Subsequent Expansion, Analysis, and Experimentation -- Gene Expression Analysis at the Single Cell Level Using the Human Bone Marrow Stromal Cell as a Model: Sample Preparation Methods -- Assays of MSCs with Microarrays -- Gene Delivery to Mesenchymal Stem Cells -- Isolation and Characterization of Murine Multipotential Stromal Cells from Bone Marrow -- Isolation of Mesenchymal Stem Cells from Murine Bone Marrow by Immunodepletion.
520			_aFor over forty years, mesenchymal stem cells (MSCs) have been scrutinized and studied, garnering much attention due to their broad therapeutic efficacy. In Mesenchymal Stem Cells: Methods and Protocols, leaders in the field were assembled to contribute detailed methodologies for the isolation and characterization of human and rodent MSCs. Recently, these vital cells have shown therapeutic benefits in the treatment of myocardial infarction, stroke, lung diseases, spinal cord injury and other neurological disorders, thus promising a boundless future in their study. Written to the specifications of the highly successful Methods in Molecular Biology™ series format, each chapter contains step-by-step laboratory protocols, a list of the necessary materials, and a Notes section with tips on troubleshooting and avoiding known pitfalls. Cutting edge and easy to use, Mesenchymal Stem Cells: Methods and Protocols is the perfect resource for scientists attempting to pursue this important and ever-developing field of research.
700	1		_aProckop, Darwin J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aPhinney, Donald G _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBunnell, Bruce A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617377679
776	0	8	_iPrinted edition: _z9781588297716
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-169-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2008
999			_c235439 _d235439