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001			235443
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008			170814s2017 xxu| s |||| 0|eng d
020			_a9781493972012
024	7		_a10.1007/978-1-4939-7201-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aProteomics for Drug Discovery _bMethods and Protocols _cedited by Iulia M. Lazar, Maria Kontoyianni, Alexandru C. Lazar.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 302 páginas) _b62 ilustraciones, 46 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1647
505	0		_aA Photoaffinity Labeling-Based Chemoproteomics Strategy for Unbiased Target Deconvolution of Small Molecule Drug Candidates -- Multiplexed Liquid Chromatography-Multiple Reaction Monitoring Mass Spectrometry Quantification of Cancer Signaling Proteins -- Monitoring Dynamic Changes of the Cell Surface Glycoproteome by Quantitative Proteomics -- High Resolution Parallel Reaction Monitoring with Electron Transfer Dissociation for Middle-Down Proteomics: An Application to Study the Quantitative Changes Induced by Histone Modifying Enzyme Inhibitors and Activators -- Preparation and Immunoaffinity Depletion of Fresh Frozen Tissue Homogenates for Mass Spectrometry-Based Proteomics in the Context of Drug Target/Biomarker Discovery -- Target Identification Using Cell Permeable and Cleavable Chloroalkane Derivatized Small Molecules -- Microfluidics-Mass Spectrometry of Protein-Carbohydrate Interactions: Applications to Development of Therapeutics and Biomarker Discovery -- Studying Protein-Protein Interactions by Biotin AP-Tagged Pulldown and LTQ-Orbitrap Mass Spectrometry -- Post-Translational Modification Profiling-Functional Proteomics for the Analysis of Immune Regulation -- Reverse Phase Protein Arrays and Drug Discovery -- Probing Protein Kinase-ATP Interactions Using a Fluorescent ATP Analog -- Preparation of Disease-Related Protein Assemblies for Single Particle Electron Microscopy -- Identification of Lipid-Binding Modulators Using the Protein-Lipid Overlay Assay -- Resazurin Live Cell Assay: Setup and Fine-Tuning for Reliable Cytotoxicity -- Exploring Protein-Protein Interactions as Drug Targets for Anti-Cancer Therapy with In Silico Workflows -- Method to Identify Silent Codon Mutations that May Alter Peptide Elongation Kinetics and Co-Translational Protein Folding -- In Silico Design of Anticancer Peptides -- Docking and Virtual Screening in Drug Discovery -- Bioinformatics Resources for Interpreting Proteomics Mass Spectrometry Data.
520			_aThis book reflects the drug development community's interest in proteomics methods by providing researchers with the necessary knowledge for the implementation of a number of basic experimental and bioinformatics protocols in the drug discovery research laboratory. The focus of the collection is on select protocols and methodologies that address the analysis of post-translational modifications, targeted protein quantification, protein-protein, protein-lipid, or protein-ATP interactions, in silico lead identification, protein arrays, tissue and cell extract preparation, labeling, chemoproteomics, and drug efficacy assessment. Emphasis was placed not just on methods that describe large-scale protein analysis, but also on ones that are amenable to future implementation in a high-throughput format. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Practical and authoritative, Proteomics for Drug Discovery: Methods and Protocols aims to provide a deep knowledge of the fundamentals of disease biology as well the means that were developed for probing the complex biological milieu.
700	1		_aLazar, Iulia M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aKontoyianni, Maria _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aLazar, Alexandru C _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493972005
776	0	8	_iPrinted edition: _z9781493972029
776	0	8	_iPrinted edition: _z9781493984145
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7201-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c235443 _d235443