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007			cr nn 008mamaa
008			100301s2008 xxu| s |||| 0|eng d
020			_a9781597452373
024	7		_a10.1007/978-1-59745-237-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aGene Therapy Protocols _bVolume 1: Production and In Vivo Applications of Gene Transfer Vectors _cedited by Joseph LeDoux.
250			_a3rd edition 2008
264		1	_aTotowa, NJ _bHumana Press _c2008
300			_a1 recurso en línea (XII, 386 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v433
505	0		_aPreparation and Quantification of Pseudotyped Retroviral Vector -- Production of Retroviral Vectors for Clinical Use -- Methods for the Production of Helper-Dependent Adenoviral Vectors -- Methods for the Production of First Generation Adenoviral Vectors -- Large-Scale Production of Recombinant Adeno-Associated Viral Vectors -- Construction and Production of Recombinant Herpes Simplex Virus Vectors -- Plasmid-Based Gene Transfer in Mouse Skeletal Muscle by Electroporation -- Chitosan Nanoparticle-Mediated Gene Transfer -- PEG-PEI Copolymers for Oligonucleotide Delivery to Cells and Tissues -- Non-Viral Gene Delivery with Cationic Liposome-DNA Complexes -- Applications of Lentiviral Vectors in Noninvasive Molecular Imaging -- Retroviral Modification of Mesenchymal Stem Cells for Gene Therapy of Hemophilia -- Transduction of Murine Hematopoietic Stem Cells and In Vivo Selection of Gene-Modified Cells -- Assessment of CFTR Function after Gene Transfer In Vitro and In Vivo -- Oncolytic Adenoviruses for Cancer Gene Therapy -- Design of Trans-Splicing Adeno-Associated Viral Vectors for Duchenne Muscular Dystrophy Gene Therapy -- Prevention of Type 1 Diabetes in NOD Mice by Genetic Engineering of Hematopoietic Stem Cells -- Lentiviral Vector Delivery of siRNA and shRNA Encoding Genes into Cultured and Primary Hematopoietic Cells -- Nanoparticle-Mediated Gene Delivery to the Lung -- Retroviral-Mediated Gene Therapy for the Differentiation of Primary Cells into a Mineralizing Osteoblastic Phenotype -- In Vivo siRNA Delivery to the Mouse Hypothalamus Shows a Role of the Co-Chaperone XAP2 in Regulating TRH Transcription -- Efficient Retroviral Gene Transfer to Epidermal Stem Cells.
520			_aIn Gene Therapy Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). The field of gene therapy has undergone remarkable advances, promising to impact human healthcare significantly in the twenty-first century. Today's technologies can deliver genetic material safely and effectively to cells to slow or halt the progression of disease, and to help repair or regenerate damaged or lost tissues. In this first volume of Gene Therapy Protocols: Production and In Vivo Applications of Gene Transfer Vectors, readers will find a comprehensive resource of current and emerging methods for the production of viral and non-viral gene transfer vectors, as well as detailed protocols for critical applications in stem cell biology, cancer, diabetes, HIV and tissue engineering. This first volume of the new and completely revised third edition of Gene Therapy Protocols will prove a necessary tool for graduate students and postdoctoral fellows and invaluable to basic and clinical researchers in both industry and academia.
700	1		_aLeDoux, Joseph _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781588299031
776	0	8	_iPrinted edition: _z9781627039567
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-237-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2008
999			_c235455 _d235455