000			03449nam a22003495i 4500
001			235457
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007			cr nn 008mamaa
008			100301s2007 xxu| s |||| 0|eng d
020			_a9781597454568
024	7		_a10.1007/978-1-59745-456-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPichia Protocols _cedited by James M. Cregg.
250			_a2nd edition 2007
264		1	_aTotowa, NJ _bHumana Press _c2007
300			_a1 recurso en línea (XII, 268 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v389
505	0		_aVectors and Strains for Expression -- DNA-Mediated Transformation -- Rational Design and Optimization of Fed-Batch and Continuous Fermentations -- Saturation of the Secretory Pathway by Overexpression of a Hookworm (Necator americanus) Protein (Na-ASP1) -- Purification of the N- and C-Terminal Subdomains of Recombinant Heavy Chain Fragment C of Botulinum Neurotoxin Serotype C -- Rapid Screening of Chromatography Resins for the Purification of Proteins -- Characterization of O-Linked Saccharides on Glycoproteins -- Modification of the N-Glycosylation Pathway to Produce Homogeneous, Human-Like Glycans Using GlycoSwitch Plasmids -- N-Linked Glycan Characterization of Heterologous Proteins -- Heavy Labeling of Recombinant Proteins -- Selenomethionine Labeling of Recombinant Proteins -- Selective Isotopic Labeling of Recombinant Proteins Using Amino Acid Auxotroph Strains -- Classical Genetics -- Identification of Pexophagy Genes by Restriction Enzyme-Mediated Integration -- Characterization of Protein-Protein Interactions -- Localization of Proteins and Organelles Using Fluorescence Microscopy -- Fluorescence Microscopy and Thin-Section Electron Microscopy.
520			_aThe use of Pichia Pastoris as a recombinant protein system has increased greatly in popularity over the years. In Pichia Protocols 2nd Edition, the author focuses on new information that has come to light in recent years but repeats key basic methods so that researchers are not absolutely dependent on having both editions in order to utilize the system. The chapters in this indispensable laboratory aid cover a wide range of topics associated with Pichia Pastoris. The use of fermentors to grow Pichia Pastoris and good advice on how to go about the initial processing of material from these cultures is discussed. An extensive discussion on how to formally analyze mutations in essential P. pastoris genes is also presented. The final chapters are for cell biologists who are for cell biologists using the Pichia Pastoris system to investigate protein interactions, or to localize proteins in yeast cells. Pichia Protocols 2nd Edition, is an essential tool for researchers working with the Pichia Pastoris system.
700	1		_aCregg, James M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617375637
776	0	8	_iPrinted edition: _z9781588294296
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-456-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2007
999			_c235457 _d235457