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008			140920s2015 xxu| s |||| 0|eng d
020			_a9781493913695
024	7		_a10.1007/978-1-4939-1369-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aRegulatory Non-Coding RNAs _bMethods and Protocols _cedited by Gordon G. Carmichael.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (X, 181 páginas) _b39 ilustraciones, 10 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1206
505	0		_aDiverse Functions and Mechanisms of Mammalian Long Non-Coding RNAs -- Sequence-Defined Oligoaminoamides for the Delivery of siRNAs -- A Biochemical Approach to Identify Direct MicroRNA Targets -- Native Gel Analysis for Mammalian MicroRNPs Assembled from Pre-MicroRNAs -- Identification and Validation of miRNA Target Sites within Non-Traditional miRNA Targets -- Fractionation of Non-Polyadenylated and Ribosomal-Free RNAs from Mammalian Cells -- Affinity-Purification of Long Noncoding RNA−Protein Complexes from Formaldehyde-Cross linked Mammalian Cells -- RNA Pull down Protocol for In Vitro Detection and Identification of RNA-Associated Proteins -- nRIP-seq: A Technique to Identify RNA Targets of an RNA Binding Protein on a Genome-Wide Scale -- Analysis of the Subcellular Distribution of RNA by Fluorescence In Situ Hybridization -- RNA Fluorescence In Situ Hybridization in Cultured Mammalian Cells -- Locating RNAs In Situ with FISH-STIC Probes -- Rapid and Efficient Elimination of Specific Nuclear Noncoding RNAs in Mammalian Cells with Antisense Oligonucleotides -- On Using Magnesium and Potassium Ions in RNA Experiments -- Studying the Affinity, Kinetic Stability and Specificity of RNA/Protein Interactions: SINE ncRNA/Pol II Complexes as a Model System.
520			_aRegulatory Non-Coding RNAs: Methods and Protocols offers a collection of methods for those interested in the discovery, localization, and functional analysis of these non-coding transcripts that have the potential and ability to orchestrate and control gene expression. After a review of the field, this detailed volume continues with methods useful for the study of siRNAs, microRNAs, and their targets, techniques concerned with long non-coding RNAs, as well as studies of the critical parameters of functional non-coding RNA protein-RNA interactions and the environment in which they act. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.   Dependable and easy to use, Regulatory Non-Coding RNAs: Methods and Protocols provides a current, state-of-the-art collection of methods and approaches that will be of value to researchers in this expanding and fascinating field.
700	1		_aCarmichael, Gordon G _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493913701
776	0	8	_iPrinted edition: _z9781493913688
776	0	8	_iPrinted edition: _z9781493941841
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1369-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c235465 _d235465