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007			cr nn 008mamaa
008			170601s2017 xxu| s |||| 0|eng d
020			_a9781493971169
024	7		_a10.1007/978-1-4939-7116-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aEbolaviruses _bMethods and Protocols _cedited by Thomas Hoenen, Allison Groseth.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XVI, 397 páginas) _b68 ilustraciones, 55 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1628
505	0		_aMarburg and Ebola Viruses: A Look Back and Lessons for the Future -- 40 Years of Ebolavirus Molecular Biology: Understanding a Novel Disease Agent through the Development and Application of New Technologies -- Ebolavirus: An Overview of Molecular and Clinical Pathogenesis -- Production of Filovirus Glycoprotein-Pseudotyped Vesicular Stomatitis Virus for Study of Filovirus Entry Mechanisms -- Lentiviral Vectors Pseudotyped with Filoviral Glycoproteins -- Modeling Ebola Virus Genome Replication and Transcription with Minigenome Systems -- Quantification of RNA Content in Reconstituted Ebola Virus Nucleocapsids by Immunoprecipitation -- Modeling Ebolavirus Budding with Virus-Like Particles -- Modeling the Ebolavirus Life Cycle with Transcription and Replication-Competent Virus-Like Particle Assays -- Assays to Measure Suppression of Type I Interferon Responses by Filovirus VP35 Proteins -- Non-Radioactive Northern Blot Analysis to Detect Ebola Virus Minigenomic mRNA -- A Semi-Automated High Throughput Microtitration Assay for Filoviruses -- Generation of Recombinant Ebola Viruses Using Reverse Genetics -- Luciferase-Expressing Ebolaviruses as Tools for Screening of Antivirals -- Live-Cell Imaging of Filoviruses -- Assessment of Inhibition of Ebola Virus Progeny Production by Antiviral Compounds -- Analysis of the Cellular Stress Response During Ebola Virus Infection by Immunofluorescence -- Analyzing Apoptosis Induction and Evasion in Ebola Virus-Infected Cells -- Electron Microscopy of Ebola Virus-Infected Cells -- Validating the Inactivation Effectiveness of Chemicals on Ebola Virus -- Visualizing Ebolavirus Particles Using Single-Particle Interferometric Reflectance Imaging Sensor (SP-IRIS) -- Assessing Antiviral Countermeasures Using Mouse Models of Ebolavirus Infection -- Evaluation of Ebola Virus Countermeasures in Guinea Pigs -- Evaluation of Medical Countermeasures against Ebolaviruses in Non-Human Primate Models -- Quantification of Filovirus Glycoprotein-Specific Antibodies -- Monitoring Innate Immune Gene Responses in the Hamster Model of Ebola Virus Disease by RT-PCR -- Real-Time and End Point PCR Diagnostics for Ebola Virus -- Production of Antigens for ELISA -- ELISA Methods for the Detection of Ebolavirus Infection -- Ebola Virus Field Sample Collection.
520			_aThis vital book collects key methods that have supported advancements in the field of ebolavirus molecular biology given the pressing need for the advancement of techniques for diagnostics, the development of vaccines and antivirals, and for furthering our understanding of ebolavirus biology. After an introduction, the volume delves into protocols for studying ebolavirus molecular biology under biosafety level 2 conditions, studying infectious ebolaviruses under biosafety level 4 conditions in vitro as well as in vivo, and working with ebolaviruses in the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Ebolaviruses: Methods and Protocols serves as a guide to the next generations of filovirus researchers and also helps to bring experts from other areas into the filovirus research arena.
700	1		_aHoenen, Thomas _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aGroseth, Allison _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493971152
776	0	8	_iPrinted edition: _z9781493971176
776	0	8	_iPrinted edition: _z9781493983896
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7116-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c235466 _d235466