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007			cr nn 008mamaa
008			151007s2015 xxu| s |||| 0|eng d
020			_a9781493929290
024	7		_a10.1007/978-1-4939-2929-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMethods in Mouse Atherosclerosis _cedited by Vicente Andrés, Beatriz Dorado.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XIV, 400 páginas) _b74 ilustraciones, 58 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1339
505	0		_aUse of Mouse Models in Atherosclerosis Research -- Cytokines and Immune Responses in Murine Atherosclerosis -- Intravital Microscopy for Atherosclerosis Research -- Non-invasive Molecular Imaging of Mouse Atherosclerosis -- Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root -- Isolation, Culture, and Polarization of Murine Bone Marrow-Derived and Peritoneal Macrophages -- Isolation of Mouse Primary Aortic Endothelial Cells by Selection with Specific Antibodies -- Isolation and Culture of Aortic Smooth Muscle Cells and In Vitro Calcification Assay -- Immunostaining of Macrophages, Endothelial Cells, and Smooth Muscle Cells in the Atherosclerotic Mouse Aorta -- Immunostaining of Lymphocytes In Mouse Atherosclerotic Plaque -- Flow Cytometric Analysis of Immune Cells Within Murine Aorta -- In Vitro Differentiation of Naïve CD4+ T cells: A Tool for Understanding the Development of Atherosclerosis -- Quantification of Apoptosis in Mouse Atherosclerotic Lesions -- Quantification of Cellular Proliferation in Mouse Atherosclerotic Lesions -- Quantification of in vitro Macrophage Cholesterol Efflux and in vivo Macrophage-Specific Reverse Cholesterol Transport -- In vitro Macrophage Phagocytosis Assay -- Glucose and Insulin Tolerance Tests in the Mouse -- Wire Myography to Study Vascular Tone and Vascular Structure of Isolated Mouse Arteries -- Generation of Aorta Transcript Atlases of Wild-type and Apolipoprotein E-null Mice by Laser Capture Microdissection-based mRNA Expression Microarrays -- Analysis of Gene and Protein Expression In Atherosclerotic Mouse Aorta By Western Blot and Quantitative Real-Time PCR -- Bone Marrow Transplantation in Mice to Study the Role of Hematopoietic Cells in Atherosclerosis -- Tandem Stenosis to Induce Atherosclerotic Plaque Instability in the Mouse -- Detection of Intraplaque Hemorrhage in Mouse Atherosclerotic Lesions -- Mechanical Stabilization of Mouse Carotid Artery for in vivo intravital Microscopy Imaging of Atherogenesis -- Intravital Microscopy in the Cremaster Muscle Microcirculation for Endothelial Dysfunction Studies. - Fluorescent Molecular Tomography for in vivo Imaging of Mouse Atherosclerosis -- In vivo 18F-FDG-PET Imaging in Mouse Atherosclerosis -- Magnetic Resonance Imaging of the Atherosclerotic Mouse Aorta.
520			_aThis volume reviews available mouse models of atherosclerosis, basic aspects of disease initiation and progression, and the visualization of atherosclerotic lesions with invasive and non-invasive techniques that are widely used both in basic research and in the clinic. The chapters catalogue a wide range of in vitro and in vivo experimental methods used for atherosclerosis research, including thorough protocols and valuable notes based on the authors' personal wet lab experience. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Methods in Mouse Atherosclerosis will be a valuable working guide for researchers performing mouse-based atherosclerosis studies.
700	1		_aAndrés, Vicente _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aDorado, Beatriz _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493929283
776	0	8	_iPrinted edition: _z9781493929306
776	0	8	_iPrinted edition: _z9781493949359
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2929-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c235467 _d235467