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| 001 | 235475 | ||
| 003 | ES-VaUE | ||
| 005 | 20221220020707.0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 100308s2010 gw | s |||| 0|eng d | ||
| 020 | _a9783642011474 | ||
| 024 | 7 |
_a10.1007/978-3-642-01147-4 _2doi |
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| 040 |
_aES-MaUEC _bspa _cES-MaUEC |
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| 245 | 1 | 0 |
_aAntibody Engineering Volume 2 _cedited by Roland E. Kontermann, Stefan Dübel. |
| 250 | _a2nd edition 2010 | ||
| 264 | 1 |
_aBerlin, Heidelberg _bSpringer International Publising _c2010 |
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| 300 |
_a1 recurso en línea (XII, 589 páginas) _b |
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| 336 |
_atexto _btxt _2rdacontent |
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| 337 |
_aelectrónico _bc _2rdamedia |
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| 338 |
_arecurso electrónico _bcr _2rdacarrier |
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| 347 |
_aarchivo de texto _bPDF |
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| 490 | 0 |
_aSpringer Protocols Handbooks _x1949-2456 |
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| 505 | 0 | _aBioinformatics of Antigen-binding Sites -- Analysis of Single Chain Antibody Sequences Using the VBASE2 Fab Analysis Tool -- Standardized Sequence and Structure Analysis of Antibody Using IMGT® -- Protein Sequence and Structure Analysis of Antibody Variable Domains -- Generation of Antibody Fragments and Their Derivatives -- scFv by Two-Step Cloning -- Bivalent Diabodies -- Generation of Single-Chain Fv Fragments and Multivalent Derivatives scFv-Fc and scFv-CH3 (Minibodies) -- Miniantibodies -- Generation of Stably Transfected Eukaryotic Cell Lines Producing ImmunoRNAse Fusion Proteins -- Antibody-Cytokine Fusion Proteins with Members of the TNF-Family -- Recombinant Immunotoxins for Treating Cancer -- T Bodies -- Expressing Intrabodies in Mammalian Cells -- Phenotypic Knockdown with Intrabodies -- Disulfide-Stabilized Fv Fragments -- PEGylation of Antibody Fragments to Improve Pharmacodynamics and Pharmacokinetics -- Fusion Proteins with Improved PK -- In Vivo Biotinylated scFv Fragments -- Bispecific Diabodies and Single-Chain Diabodies -- Generation and Characterization of a Dual Variable Domain Immunoglobulin (DVD-Ig™) Molecule -- Isolation of Antigen-Specific Nanobodies -- CDR-FR Peptides -- Production of Antibody Fragments -- Purification and Characterization of His-Tagged Antibody Fragments -- Production of Antibody Fragments in the Gram-Positive Bacterium Bacillus megaterium -- Analysis and Purification of Antibody Fragments Using Protein A, Protein G, and Protein L -- Purification and Analysis of Strep-tagged Antibody-Fragments -- Production of Antibodies and Antibody Fragments in Escherichia coli -- Improving Expression of scFv Fragments by Co-expression of Periplasmic Chaperones -- Bioreactor Production of scFv Fragments in Pichia pastoris -- Expression of Antibody Fragments in Transgenic Plants -- Transient Production of scFv-Fc Fusion Proteins in Mammalian Cells -- Recombinant Antibody Molecules in Nanobiotechnology and Proteomics -- Immunoliposomes -- Targeted Polymeric Nanoparticles -- Antibody Microarrays for Expression Analysis -- Evaluation of Recombinant Antibodies on Protein Microarrays Applying the Multiple Spotting Technique -- Preclinical and Clinical Development -- Xenograft Mouse Models for Tumour Targeting -- Xenograft Mouse Models for Tumour Targeting -- Imaging Tumor Xenografts Using Radiolabeled Antibodies -- Human Anti-antibody Response -- IP Issues in the Therapeutic Antibody Industry. | |
| 520 | _aAntibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. Antibody Engineering provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest "hands on" knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include helpful practical advice; experienced antibody engineers will appreciate the new ideas and approaches. The book is an invaluable resource for all those engaged in antibody research and development. | ||
| 700 | 1 |
_aKontermann, Roland E _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt |
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| 700 | 1 |
_aDübel, Stefan _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt |
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| 776 | 0 | 8 |
_iPrinted edition: _z9783642011481 |
| 776 | 0 | 8 |
_iPrinted edition: _z9783642422768 |
| 776 | 0 | 8 |
_iPrinted edition: _z9783642011467 |
| 856 | 4 | 0 |
_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-3-642-01147-4 _z(usuarios Universidad Europea de Valencia) |
| 942 |
_2lcc _cLE |
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| 988 | _aSpringer_Protocols_2010 | ||
| 999 |
_c235475 _d235475 |
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