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008			170308s2017 xxu| s |||| 0|eng d
020			_a9781493968244
024	7		_a10.1007/978-1-4939-6824-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMitochondria _bPractical Protocols _cedited by Dejana Mokranjac, Fabiana Perocchi.
250			_a2nd edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 409 páginas) _b77 ilustraciones, 46 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1567
505	0		_aA Guide to Computational Methods for Predicting Mitochondrial Localization -- Isolation of Functional Mitochondria from Cultured Cells and Mouse Tissues -- Isolation of Mitochondria From Saccharomyces Cerevisiae -- Isolation of Contact Sites Between Inner and Outer Mitochondrial Membranes -- Isolation Of Mitochondria-Associated Membranes (MAM) from Mouse Brain Tissue -- Label-Free Quantitative Analysis of Mitochondrial Proteomes using the Multienzyme Digestion-Filter Aided Sample Preparation (MED-FASP) and 'Total Protein Approach' -- Quantitative Analysis of Glycerophospholipids in Mitochondria by Mass Spectrometry -- Detection of Cysteine Redox States in Mitochondrial Proteins in Intact Mammalian Cells -- Chemical Crosslinking in Intact Mitochondria -- Reconstitution of Mitochondrial Membrane Proteins into Nanodiscs by Cell-Free Expression -- Detection of Dual Targeting and Dual Function of Mitochondrial Proteins in Yeast -- Localizing Mrnas Encoding Mitochondrial Proteins in Yeast by Fluorescence Microscopy and Subcellular Fractionation -- Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Various Mouse Tissues using Seahorse XF96 Analyzer -- Application of FRET-Based Biosensor "Ateam" for Visualization of ATP Levels in the Mitochondrial Matrix of Living Mammalian Cells -- A Microplate-Based Bioluminescence Assay of Mitochondrial Calcium Uptake -- New Imaging Tools to Analyze Mitochondrial Morphology in Caenorhabditis Elegans -- Single Molecule Tracking and Localization Of Mitochondrial Protein Complexes in Live Cells -- Analysis of Yeast Mitochondria by Electron Microscopy -- Analysis of Mitochondrial Membrane Protein Complexes by Electron Cryo-Tomography -- Assays for Mitophagy in Yeast -- Assessing Mitochondrial Selective Autophagy in the Nematode Caenorhabditis Elegans -- Assessing Mitochondrial Unfolded Protein Response in Mammalian Cells -- Analysis of Mitochondrial RNA-Processing Defects in Patient-Derived Tissues by Qrt-PCR and Rnaseq -- Identification of Disease-Causing Mutations by Functional Complementation of Patient-Derived Fibroblast Cell Lines. .
520			_aThis volume compiles a broad range of step-by-step protocols, complementary to the ones published in the first edition of this book, to study various aspects of mitochondrial structure and function in different model organisms, both in vitro and in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mitochondria: Practical Protocols, Second Edition aims to be useful for beginners as well as for experienced researchers in the field.
700	1		_aMokranjac, Dejana _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aPerocchi, Fabiana _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493968220
776	0	8	_iPrinted edition: _z9781493968237
776	0	8	_iPrinted edition: _z9781493983094
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6824-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c235482 _d235482