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| 001 | 235485 | ||
| 003 | ES-VaUE | ||
| 005 | 20221220020708.0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 100301s2002 xxu| s |||| 0|eng d | ||
| 020 | _a9781592591794 | ||
| 024 | 7 |
_a10.1385/1592591795 _2doi |
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| 040 |
_aES-MaUEC _bspa _cES-MaUEC |
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| 245 | 1 | 0 |
_aIn Situ Detection of DNA Damage _bMethods and Protocols _cedited by Vladimir V. Didenko. |
| 250 | _a1st edition 2002 | ||
| 264 | 1 |
_aTotowa, NJ _bHumana Press _c2002 |
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| 300 |
_a1 recurso en línea (XVII, 313 páginas) _b164 ilustraciones, 31 ilustraciones a color |
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| 336 |
_atexto _btxt _2rdacontent |
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| 337 |
_aelectrónico _bc _2rdamedia |
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| 338 |
_arecurso electrónico _bcr _2rdacarrier |
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| 347 |
_aarchivo de texto _bPDF |
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| 490 | 0 |
_aMethods in Molecular Biology _x1940-6029 _v203 |
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| 505 | 0 | _aLabeling DNA Breaks Using Terminal Transferase (TUNEL Assay) -- Labeling DNA Damage with Terminal Transferase -- TUNEL Assay -- Electron Microscopic Detection of DNA Damage Labeled by TUNEL -- Quantitative Differentiation of Both Free 3? OH and 5? OH DNA Ends Using Terminal Transferase-Based Labeling Combined with Transmission Electron Microscopy -- Determination of Three-Dimensional Distribution of Apoptotic DNA Damage by Combination of TUNEL and Quick-Freezing and Deep-Etching Techniques -- In Situ Detection of DNA Strand Breaks in Analysis of Apoptosis by Flow- and Laser-Scanning Cytometry -- Labeling DNA Breaks Using DNA Polymerase I or its Klenow Fragment -- DNA Damage Detection Using DNA Polymerase I or its Klenow Fragment -- Labeling DNA Breaks In Situ by Klenow Enzyme -- In Situ Nick Translation at the Electron Microscopic Level -- Labeling DNA Breaks Using Ligase -- In Situ DNA Ligation as a Method for Labeling Apoptotic Cells in Tissue Sections -- Detection of Specific Double-Strand DNA Breaks and Apoptosis In Situ Using T4 DNA Ligase -- In Situ Detection of Double-Strand DNA Breaks with Terminal 5?OH Groups -- Detection of DNA Breaks in Agarose Trapped Cells: Comet Assay and Related Techniques -- The Comet Assay -- The Comet Assay -- Ultrasensitive Detection of DNA Damage by the Combination of the Comet and TUNEL Assays -- Application of FISH to Detect DNA Damage -- Detection of Modified Bases and AP Sites in DNA -- Simultaneous In Situ Detection of DNA Fragmentation and RNA/DNA Oxidative Damage Using TUNEL Assay and Immunohistochemical Labeling for 8-Hydroxy-2?-Deoxyguanosine (8-OHdG) -- The In Situ Detection of Apurinic/Apyrimidinic Sites and DNA Breaks Bearing Extension Blocking Termini -- Indirect and General Markers of DNA Damage -- Markers of Poly (ADP-Ribose) Polymerase Activity as Correlates of DNA Damage -- Ultrasound Imaging of Apoptosis -- p53 Induction as an Indicator of DNA Damage -- Detection of Caspases Activation In Situ by Fluorochrome-Labeled Inhibitors of Caspases (FLICA). | |
| 520 | _aDetection and analysis of DNA damage is critically important in the study of the widest variety of biological phenomena, ranging from apoptosis and aging to heart diseases and anticancer drug development. In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and show how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. In-depth protocols for single-cell electrophoresis (comet assay) are presented, as well as those for the detection of indirect markers of DNA damage and modified bases. Technical reviews discuss specificity, sensitivity, advantages, and limitations of the techniques described, and compare them with alternative approaches. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying not only the in situ detection of DNA damage but also the labeling of apoptotic cells. | ||
| 700 | 1 |
_aDidenko, Vladimir V _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt |
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| 776 | 0 | 8 |
_iPrinted edition: _z9781617372728 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781489928085 |
| 776 | 0 | 8 |
_iPrinted edition: _z9780896039520 |
| 856 | 4 | 0 |
_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592591795 _z(usuarios Universidad Europea de Valencia) |
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_2lcc _cLE |
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| 988 | _aSpringer_Protocols_2002 | ||
| 999 |
_c235485 _d235485 |
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